DEVELOPMENT OF A RADIOIMMUNOASSAY FOR HUMAN GROUP-II PHOSPHOLIPASE-A(2) AND DEMONSTRATION OF POSTOPERATIVE ELEVATION
DEVELOPMENT OF A RADIOIMMUNOASSAY FOR HUMAN GROUP-II PHOSPHOLIPASE-A(2) AND DEMONSTRATION OF POSTOPERATIVE ELEVATION
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DOI：
10.1159/000468890
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发表时间：
1991-01-01
期刊：
影响因子：
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通讯作者：
MORI, T
中科院分区：
文献类型：
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作者：
MATSUDA, Y;OGAWA, M;MORI, T
建立了测定人第II组磷脂酶A2(M-PLA2)的放射免疫分析方法。M-PLA2是从人脾中提纯的。将免疫小鼠的脾细胞与M-PLA2和小鼠骨髓瘤细胞系NS-1融合，制备单抗。放射免疫分析采用单抗方法。该方法灵敏(0.78mU·g/L)，重现性好，特异性强。健康人血清M-PLA2浓度在1.4~4.2mU/g/L之间，平均值为2.2mU/g/L(平均值+/-SE)。应用放射免疫法，我们发现各种感染和恶性肿瘤患者的血清M-PLA2升高。在无任何感染并发症的病例中，术后一过性血清M-PLA2升高。手术高度与手术方式或手术部位无关。术后第2~4天血清M-PLA2水平最高。在这些患者中，血清M-PLA2和C-反应蛋白水平显著相关。本研究表明，血清M-PLA2为急性时相反应物。
A radioimmunoassay (RIA) for the determination of human group-II phospholipase A2 (M-PLA2) has been developed. M-PLA2 was purified from human spleen. Monoclonal antibody (IgG) was prepared by fusion of splenic cells from immunized mice with M-PLA2 and the mouse myeloma cell line NS-1. The RIA was carried out by a single antibody method. The assay is sensitive (0.78-mu-g/l), reproducible and specific. In healthy individuals, the serum M-PLA2 concentration ranges from 1.4 to 4.2-mu-g/l, the average being 2.2 +/- 0.1-mu-g/l (mean +/- SE). Using the RIA, we found increased serum M-PLA2 in patients with various infections and malignant tumors. We also showed the postoperative transient elevation of serum M-PLA2 in cases without any infectious complications. The elevation was independent of the surgical procedure or site. The maximum serum M-PLA2 level was seen on the 2nd to 4th postoperative day. In these patients, the serum M-PLA2 and C-reactive protein levels were significantly correlated. The present study indicated that serum M-PLA2 is an acute phase reactant.