Immunological Studies on the Influence of Chromatin Structure on the Binding of a Chemical Carcinogen to the Genome
Immunological Studies on the Influence of Chromatin Structure on the Binding of a Chemical Carcinogen to the Genome
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染色质结构对化学致癌物与基因组结合影响的免疫学研究
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
M. Seidman
中科院分区：
文献类型：
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作者：
M. Bustin;P. Kurth;H. Slor;M. Seidman
研究了染色质结构对苯并（a）芘代谢物与基因组结合的影响。用[r-7，t-8-dihydroxy-t-9，10-oxy-7，8，9，10-tetrahydrobenzo（a）pyrene] BPDE修饰的DNA引发的抗体检测细胞染色质、SV 40限制性片段和多线染色体中的致癌物修饰区。已经开发了一种方法，该方法允许检测来自DNA分子的限定限制性片段中的加合物，该DNA分子被修饰为每个DNA分子少于一个致癌物加合物，多烯染色体允许直接可视化致癌物结合区域。3 H-BPDE处理的染色体的放射自显影，然后在存在或不存在RNase的情况下进行免疫荧光，可以区分与DNA、RNA和蛋白质的结合。结果表明，染色质的核小体结构对致癌物的结合有很小的影响。然而，多线染色体中的某些位点显示出抗BPDE结合的显著偏好。
The influence of chromatin structure on the binding of benzo- (a)pyrene metabolites to the genome is studied. Carcinogen modified regions, in cellular chromatin, in restriction fragments of SV40, and in polytene chromosomes are detected using antibodies elicited by [r-7, t-8-dihydroxy-t-9, 10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene] BPDE-modified DNA. A method has been developed which allows detection of adducts in defined restriction fragments derived from DNA molecules modified to less than one carcinogen adduct per DNA molecule, Polytene chromosomes allow direct visualization of carcinogen binding regions. Autoradiography of 3H-BPDE-treated chromosomes followed by immunofluorescence in the presence or absence of RNase allows distinction between the binding to DNA, RNA, and proteins. The results indicate that the nucleosomal structure of chromatin has a very slight effect on the binding of the carcinogen. However, certain loci in the polytene chromosomes display a significant preference for anti-BPDE binding.