Transcription profiling of eleven cytochrome P450s potentially involved in xenobiotic metabolism in the mosquito Aedes aegypti
Transcription profiling of eleven cytochrome P450s potentially involved in xenobiotic metabolism in the mosquito Aedes aegypti
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DOI：
10.1111/j.1365-2583.2009.00967.x
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发表时间：
2010-04-01
影响因子：
2.6
通讯作者：
Reynaud, S.
中科院分区：
文献类型：
--
作者：
Poupardin, R.;Riaz, M. A.;Reynaud, S.
研究了11个可能参与外源代谢的埃及伊蚊CYP6和CYP9亚家族P450基因的转录谱。许多基因在典型的参与外源代谢的组织中优先转录，包括中肠和马氏小管。生命阶段转录谱分析揭示了幼虫、蛹和成年雄性和雌性之间的重要差异。暴露于亚致死剂量的三种外源药物后，蚊子幼虫可诱导多个基因的转录，并在暴露48 ~ 72 h后出现诱导峰。几个CYP基因也被氧化应激诱导，其中一个基因对20-羟基蜕皮激素反应强烈。总的来说，这项研究揭示了这些p450根据外源暴露、生命阶段或性别表现出不同的转录谱。讨论了它们可能的化学保护功能。
Transcription profiles of 11 Aedes aegypti P450 genes from CYP6 and CYP9 subfamilies potentially involved in xenobiotic metabolism were investigated. Many genes were preferentially transcribed in tissues classically involved in xenobiotic metabolism including midgut and Malpighian tubules. Life-stage transcription profiling revealed important variations amongst larvae, pupae, and adult males and females. Exposure of mosquito larvae to sub-lethal doses of three xenobiotics induced the transcription of several genes with an induction peak after 48 to 72 h exposure. Several CYP genes were also induced by oxidative stress and one gene strongly responded to 20-hydroxyecdysone. Overall, this study revealed that these P450s show different transcription profiles according to xenobiotic exposures, life stages or sex. Their putative chemoprotective functions are discussed.