S100 proteins in Corpora Amylacea from normal human brain
S100 proteins in Corpora Amylacea from normal human brain
复制标题
DOI：
10.1016/s0006-8993(00)02393-3
复制
发表时间：
2000-06-09
期刊：
影响因子：
2.9
通讯作者：
Pochet, R
中科院分区：
文献类型：
--
作者：
Hoyaux, D;Decaestecker, C;Pochet, R
淀粉体（C.A.）也称为聚葡聚糖体（P.B.）是正常大脑衰老的标志之一。虽然它们的功能还不清楚。 C.A.患有神经退行性疾病的患者数量增加。 C.A.含有88%的己糖和4%的蛋白质。 C.A. 中的大部分蛋白质是老化或应激蛋白，例如热休克蛋白、泛素化蛋白和晚期糖基化终产物，它们也是促炎产物。受某些 S100 蛋白在 C.A. 可能引发的炎症过程中所发挥的潜在规则的刺激，我们通过免疫组织化学研究了不同 S100 蛋白（S100A1、S100A2、S100A3、S100A4、S100A5、S100A6、S100A8；S100A9、S100A12 和S100B）在加利福尼亚州；来自正常人的大脑。在分析的 10 种 S100 蛋白中，在 C.A. 中检测到了 9 种 (S100A)。在 C.A. 中检测到三种 S100 蛋白（S100A8、S100A9、S100A12），它们在活化的巨噬细胞中高表达并用作炎症标志物。此外，在猪栏的厚神经元突起中也发现了 S100A8。在 C.A. 中找不到一台 (S100B)尽管它在星形胶质细胞中高度表达。在 C.A.. 中，通过计算机辅助显微镜估计染色强度并给出：以下顺序：S100A1 等于 S100A8 等于 S100A9>S1005 大于或等于 S100A4>S100A12>S100A6>S100A2=S100A3。 S100 蛋白在 C.A. 中发挥的潜在炎症作用正在讨论。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Corpora amylacea (C.A.) also named polyglucosan bodies (P.B.) are one of the hallmarks of normal brain aging. Although their functions are not yet clear. C.A. increase in number in patients suffering from neurodegenerative diseases. C.A. contain 88% of hexoses and 4% of proteins. Most of the proteins in C.A. are aging or stress proteins such as heat shock proteins, ubiquitinated proteins and advanced glycation end products which are also proinflammatory products. Stimulated by the potential rule played by some S100 proteins in the inflammatory process which may be triggered in C.A., we investigated, by immunohistochemistry, the presence of different S100 proteins (S100A1. S100A2, S100A3, S100A4, S100A5, S100A6, S100A8; S100A9, S100A12 and S100B) in C.A; from normal human brain. Among the ten S100 proteins analyzed, nine (S100A) were detected in C.A. Three S100 proteins (S100A8, S100A9, S100A12) which are highly expressed in activated macrophages and used as inflammatory markers were detected in C.A. S100A8 was, in addition, found in thick neuronal processes from the pens. One (S100B) could not be found in C.A. although it was highly expressed in astrocytes. In C.A.. the staining intensity was estimated by computer-assisted microscopy and gave th; following order: S100A1 congruent to S100A8 congruent to S100A9>S1005 greater than or equal to S100A4>S100A12>S100A6>S100A2=S100A3. The potential inflammatory role-played by S100 proteins in C.A. is discussed. (C) 2000 Elsevier Science B.V. All rights reserved.