Sizing silver nanoparticles in chicken meat using direct slurry sampling graphite furnace atomic absorption spectrometry
Sizing silver nanoparticles in chicken meat using direct slurry sampling graphite furnace atomic absorption spectrometry
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DOI：
10.1007/s00216-019-01606-7
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发表时间：
2019-07-01
影响因子：
4.3
通讯作者：
Leopold, Kerstin
中科院分区：
文献类型：
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作者：
Brucker, Dominic;Leopold, Kerstin
最近，石墨炉原子吸收光谱法（GFAAS）被认为是金属纳米颗粒（NPs）检测和测定的一种工具，它具有直接分析固体样品、高样品通量、可靠和经济的仪器等优点。为此，对新引入的吸光度信号标准进行评估，即雾化延迟（t(ad)）和雾化速率（k(at)）。然而，在实际样品中，NPs通常由工程涂层试剂或天然材料稳定，并且以未知的浓度出现。因此，系统地研究了九种不同的涂层试剂和银浓度对银纳米粒子（AgNPs）雾化行为的可能影响。对吸收信号特性的评估表明，涂层和银浓度对观测参数没有影响。此外，尺寸相关的测量给出了独立于涂层的可重复的尺寸相关性。通过对两种参考材料的研究，成功地证明了该方法对AgNPs分级的有效性。在RM 8017 （NIST）中发现的74.35.9nm尺寸与认证的74.6 +/- 3.8nm尺寸非常吻合。此外，在基质参比物质nanolyse13中，加入pvp - agnps -的鸡肉匀浆中，通过直接浆液进样GFAAS发现AgNP尺寸为25.1 +/- 2.5nm，与TEM测定的27.3 +/- 5.3nm的参考值非常吻合。
Recently, graphite furnace atomic absorption spectrometry (GFAAS) has been suggested as a tool for detection and sizing of metal nanoparticles (NPs) providing several advantages, such as direct analysis of solid samples, high sample throughput, and robust and cost-efficient instrumentation. For this purpose, evaluation of newly introduced criteria of the absorbance signal, namely, atomization delay (t(ad)) and atomization rate (k(at)), is performed. However, in real samples, NPs are typically stabilized by either engineered coating reagents or natural materials and occur in unknown concentration. Hence, systematic investigation of possible influences of nine different coating reagents and of Ag concentration on the atomization behavior of silver nanoparticles (AgNPs) was studied. Evaluation of absorption signal characteristics revealed no influence of the coating or Ag concentration on the observed parameters. Furthermore, size-dependent measurements gave reproducible size correlation independent from the coating. Validity of sizing AgNPs with the proposed approach was successfully proven by investigation of two reference materials. The found size of 74.35.9nm in RM 8017 (NIST) agrees very well with the certified size of 74.6 +/- 3.8nm. Moreover, AgNP size of 25.1 +/- 2.5nm found by direct slurry sampling GFAAS in matrix reference material NanoLyse13-chicken meat homogenate spiked with PVP-AgNPs-was in very good agreement with the reference value of 27.3 +/- 5.3nm as determined by TEM.