Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus
Cell and nucleus deformation in compressed chondrocyte-alginate constructs: temporal changes and calculation of cell modulus
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DOI：
10.1016/s0304-4165(02)00144-7
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发表时间：
2002-02-15
影响因子：
3
通讯作者：
Bader, DL
中科院分区：
文献类型：
--
作者：
Knight, MM;Bravenboer, JVDB;Bader, DL
机械负荷对于关节软骨的稳态是必不可少的，并且对于使用接种在支架（例如藻酸盐）中的分离的细胞实现功能性组织工程软骨修复可能是必要的。软骨细胞的机械转导知之甚少，但可能涉及细胞变形和相关的细胞内细胞器的扭曲。本研究采用共聚焦显微镜检查细胞和细胞核形态在孤立的软骨细胞压缩藻酸盐结构。2%藻酸盐的压缩导致细胞从球形变形为扁椭圆形形态，细胞体积保持不变。细胞变形与变形有关，在较小程度上，核。尽管恒定的细胞变形超过25分钟的静态压缩，核变形显着减少，特别是在垂直于所施加的压缩轴。由较低藻酸盐浓度制成的构建体表现出降低的压缩模量，并改变了细胞对压缩的反应。在1.2%藻酸盐中，压缩导致细胞变形，其最初与2%藻酸盐中的细胞变形具有相似的幅度，但随后在60分钟内减少，反映了凝胶的粘弹性行为。这种现象使得能够计算具有约100%的估计杨氏模量值的电池的应力-应变关系。3千帕。(C)2002 Elsevier Science B. V.保留所有权利。
Mechanical loading is essential for the homeostasis of articular cartilage and may be necessary for achieving functional tissue engineered cartilage repair using isolated cells seeded in scaffolds such as alginate. Chondrocyte mechanotransduction is poorly understood, but may involve cell deformation and associated distortion of intracellular organelles. The present study used confocal microscopy to examine cell and nucleus morphology in isolated chondrocytes compressed in alginate constructs. Compression of 2%,, alginate resulted in cell deformation from a spherical to an oblate ellipsoid morphology with conservation of cell volume. Cell deformation was associated with deformation, to a lesser degree, of the nucleus. Despite constant cell deformation over a 25 min period of static compression, the nucleus deformation reduced significantly, particularly in the axis perpendicular to the applied compression. Constructs made of a lower alginate concentration exhibited a reduced compressive modulus with an altered cellular response to compression. In 1.2% alginate, compression resulted in cell deformation which was initially of a similar magnitude to that in 2%,, alginate but subsequently reduced over a 60 min period reflecting the viscoelastic behaviour of the gel. This phenomenon enabled the calculation of a stress-strain relationship for the cell with an estimated Young's modulus value of approx. 3 kPa. (C) 2002 Elsevier Science B.V. All rights reserved.