LOCAL CEREBRAL GLUCOSE-UTILIZATION IN THE FREE MOVING MOUSE - A COMPARISON DURING 2 STAGES OF THE ACTIVITY REST CYCLE
LOCAL CEREBRAL GLUCOSE-UTILIZATION IN THE FREE MOVING MOUSE - A COMPARISON DURING 2 STAGES OF THE ACTIVITY REST CYCLE
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DOI：
10.1016/0006-8993(85)91129-1
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发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
DESROSIERS, MH
中科院分区：
文献类型：
--
作者：
JAY, TM;JOUVET, M;DESROSIERS, MH
应用2-脱氧-D [1- 14 C]葡萄糖（[14 C]DG）技术对自由活动小鼠进行了局部脑葡萄糖利用（LCGU）的定量测定。获得了LCGU的可再现值，表明[14 C]DG方法具有足够的分辨率，可以对非常小的结构进行可视化和定量，前提是在微量样品、从适当组织切片制备的放射自显影和用于分析由此获得的图谱的适当技术上测量葡萄糖和[14 C]DG血浆浓度。在光暗周期的两个阶段期间，在自由移动的小鼠中测量LCGU，一个阶段对应于休息期，另一个阶段对应于高运动活动期。在两组动物中，LCGU在灰质中是异质的，在听觉区域、小脑和前庭核中发现最高值。结果表明，白天嗜睡组LCGU明显低于夜间活动组，且在感觉运动皮质、隔核、嗅束核、杏仁基底核、丘脑腹侧核、外侧膝状体、内侧膝状体和听觉皮质等8个结构中差异显著。相反，视交叉上核在白天非常活跃，在夜间相对不活跃，如先前在大鼠中报道的。
The 2-deoxy-D[1-14C]glucose ([14C]DG) technique has been applied to the free moving mouse for the quantitative determination of local cerebral glucose utilization (LCGU). Reproducible values for LCGU were obtained indicating that the [14C]DG method had a sufficient resolution power to allow visualization and quantification of very small structures provided that glucose and [14C]DG plasma concentrations were measured on microsamples, autoradiographs prepared from proper tissue sections and suitable techniques used for analysis of the maps thus obtained. LCGU was measured in free moving mice during two stages of the light-dark cycle, one corresponding to a period of rest and the other to a period of high motor activity. In the two groups of animals LCGU was heterogeneous in the grey matter, the highest values being found in the auditory regions, the cerebellar and vestibular nuclei. LCGU was found to be lower in drowsy animals during the day than in active animals during the night and the difference was significant in the 8 following structures: the sensorimotor cortex, the septal nuclei, the nucleus of the olfactory tract, the basal amygdaloid nucleus, the ventral nucleus of the thalamus, the lateral geniculate body, the medial geniculate body and the auditory cortex. On the contrary, the suprachiasmatic nucleus was very active during the day and relatively inactive during the night as previously reported in the rat.