STRUCTURE OF BLUETONGUE VIRUS CAPSID
STRUCTURE OF BLUETONGUE VIRUS CAPSID
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DOI：
10.1128/jvi.10.4.783-794.1972
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发表时间：
1972-01-01
影响因子：
5.4
通讯作者：
ELS, HJ
中科院分区：
文献类型：
--
作者：
VERWOERD, DW;HUISMANS, H;ELS, HJ
在蓝舌病病毒（BTV）的衣壳中发现了7种多肽，其中4种是主要成分，3种是次要成分。这种数量和大小分布与呼肠孤病毒相同，后者具有类似的分节双链核糖核酸基因组。在这两种病毒中，在某些基因组片段的分子量和多肽的分子量之间发现了极好的相关性，这表明它们之间存在直接的编码关系。然而，仔细比较表明，这两种病毒的相关模式是不同的，这表明不同的基因组片段可能被用来编码某些衣壳蛋白。这种现象可能与这些病毒在形态上可以区分有关，呼肠孤病毒具有较大的直径和双层衣壳，而BTV具有较小的单层衣壳。然而，利用CsCl梯度的降解作用，证明了两种BTV多肽作为衣壳周围的弥漫性蛋白质层存在。这个外层可能具有呼肠孤病毒外衣壳的一些功能，其中之一是病毒转录酶的“掩蔽”，这在去除后可以证明。
Seven polypeptides were found to be present in the capsid of the bluetongue virus (BTV), four of which are major and three are minor components. This number and size distribution is the same as that found in reovirus, which has a similar segmented, double-stranded ribonucleic acid genome. In both viruses an excellent correlation is found between the molecular weights of certain genome segments and those of the polypeptides, suggesting a direct coding relationship between them. Careful comparison, however, indicates that the correlation patterns of the two viruses are different, suggesting that different genome segments may be utilized to code for certain capsid proteins. This phenomenon may be related to the fact that these viruses can be distinguished morphologically, reovirus having a larger diameter and possessing a double-layered capsid against the smaller single capsid of the BTV. Utilizing the degrading effect of CsCl gradients, it was demonstrated, however, that two of the BTV polypeptides are present as a diffuse protein layer surrounding the capsid. This outer layer probably has some of the functions of the reovirus outer capsid, one being the “masking” of a viral transcriptase which could be demonstrated after its removal.