Effects of urea and cyanate on sickling in vitro.
Effects of urea and cyanate on sickling in vitro.
复制标题
尿素和氰酸盐对体外镰状化的影响。
DOI：
10.1056/nejm197207132870201
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
D. Nathan
中科院分区：
文献类型：
--
作者：
G. Segel;S. Feig;W. Mentzer;R. Mccaffrey;R. Wells;H. Bunn;S. Shohet;D. Nathan
摘要将SS患者的红细胞在低氧条件下培养，以比较尿素和氰酸盐对镰状化现象的影响。在尿素浓度为0.2 M（BUN，560 mg/100 ml）时，未显示镰状化抑制。用于进一步研究的镰状化率的一个更敏感的指数涉及测量在缺氧环境中经历镰状化的镰状细胞的钾损失率。而20毫米CNO-显着抑制镰状和钾的损失下3%的氧气，钾的损失仅略有减少0.2 M尿素，并没有显着减少0.1 M尿素（BUN，280毫克每100毫升）。在其他研究中，血红蛋白S的氧亲和力被0.4 M尿素（BUN，1120 mg/100 ml）增加，但不被0.1 M尿素增加。低氧诱导的高粘血症的镰状血只有轻微下降0.1 M尿素。因此，尿素似乎有抑制镰状化的作用，但只有在浓度为100 μ g/ml时才有。
Abstract Erythrocytes from patients with SS disease were incubated under reduced oxygen tension to determine the comparative effects of urea and cyanate upon the sickling phenomenon. At a urea concentration of 0.2 M (BUN, 560 mg per 100 ml), no inhibition of sickling could be demonstrated. A more sensitive index of the rate of sickling used for further studies involved measurement of the rate of potassium loss from sickle cells undergoing sickling in a hypoxic environment. Whereas 20 mM CNO- markedly inhibited sickling and potassium loss under 3 per cent oxygen, potassium loss was only slightly decreased by 0.2 M urea and was not significantly reduced by 0.1 M urea (BUN, 280 mg per 100 ml). In other studies, oxygen affinity of hemoglobin S was increased by 0.4 M urea (BUN, 1120 mg per 100 ml) but not by 0.1 M urea. Hypoxia-induced hyperviscosity of sickle blood was only slightly decreased by 0.1 M urea. Thus, urea appears to have an inhibitory effect on sickling in vitro but only at concentrations exceedi...