Singular Behavior of Slow Dynamics of Single Excitable Cells
Singular Behavior of Slow Dynamics of Single Excitable Cells
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DOI：
10.1529/biophysj.108.139691
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发表时间：
2009-01-07
影响因子：
3.4
通讯作者：
Kori, Hiroshi
中科院分区：
文献类型：
--
作者：
Harada, Takahiro;Yokogawa, Tomomi;Kori, Hiroshi
在各种培养的细胞中，已经报道了膜电位表现出具有长期相关性的波动，尽管其潜在的机制仍有待阐明。心肌细胞培养作为一个很好的实验系统来研究这种现象，因为可以通过收缩的可视化在一个延长的时间段内以非侵入性的方式来确定兴奋的时间，虽然在单细胞水平上的心肌细胞的搏动定时波动的性质仍有待充分澄清。在这篇文章中，我们报告了我们的调查培养的大鼠心肌细胞在单细胞水平的自发收缩。结果发现，单细胞表现出几种典型的收缩和自发转换的时间模式。对心搏间期时间序列的去趋势波动分析显示，在足够大的时间尺度上存在1/f（β）噪声。此外，在心搏间期时间序列中也发现了多重分形现象。这些实验趋势成功地解释了使用一个简单的数学模型，将相关噪声到离子电流。根据这些发现，可以确定伴随1/f（beta）噪声和多重分形的奇异波动是单个心肌细胞的内在特性。
In various kinds of cultured cells, it has been reported that the membrane potential exhibits fluctuations with long-term correlations, although the underlying mechanism remains to be elucidated. A cardiac muscle cell culture serves as an excellent experimental system to investigate this phenomenon because timings of excitations can be determined over an extended time period in a noninvasive manner through visualization of contractions, although the properties of beat-timing fluctuations of cardiac muscle cells at the single-cell level remains to be fully clarified. In this article, we report on our investigation of spontaneous contractions of cultured rat cardiac muscle cells at the single-cell level. It was found that single cells exhibit several typical temporal patterns of contractions and spontaneous transitions among them. Detrended fluctuation analysis on the time series of interbeat intervals revealed the presence of 1/f(beta) noise at sufficiently large timescales. Furthermore, multifractality was also found in the time series of interbeat intervals. These experimental trends were successfully explained using a simple mathematical model, incorporating correlated noise into ionic currents. From these findings, it was established that singular fluctuations accompanying 1/f(beta) noise and multifractality are intrinsic properties of single cardiac muscle cells.