Influence of environmental storage relative humidity on biological indicator resistance, viability, and moisture content
Influence of environmental storage relative humidity on biological indicator resistance, viability, and moisture content
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环境储存相对湿度对生物指示剂抗性、活力和水分含量的影响
DOI：
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发表时间：
1982
影响因子：
4.4
通讯作者：
L. L. Morien
中科院分区：
文献类型：
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作者：
R. Reich;L. L. Morien
评价了环境储存相对湿度（RH）对生物指示剂（BI）的含水量、活力、湿热和气态环氧乙烷（EO）耐受性的影响。初始嗜热脂肪芽孢杆菌孢子种群与在20 ℃和0、20、44、55%RH或环境、4 ℃或-20 ℃条件下储存12个月后的BI孢子种群之间未观察到统计学显著差异。发现在20 ℃和0或20%RH下储存的BI的耐湿热性从初始起始水平出现统计学显著性下降。B有统计学显著性降低。与初始水平相比，当BI储存在20 ℃和0% RH下时，枯草芽孢杆菌BI孢子数量显著增加，同时其EO抗性显著增加。BI在20 ℃和20或44%RH下储存，或在环境温度、4 ℃或-20 ℃条件下储存，对EO抗性无显著影响。与起始水平相比，在20 ℃和66% RH下储存的BI显示出显著较低的EO抗性。
The effect of environmental storage relative humidity (RH) on the moisture content, viability, and moist heat and gaseous ethylene oxide (EO) resistance of biological indicators (BIs) was evaluated. No statistically significant difference was observed between the initial Bacillus stearothermophilus spore population and the spore population of BIs stored at 20 degrees C and 0, 20, 44, of 55% RH or under ambient, 4 degrees C, or -20 degrees C conditions after 12 months. A statistically significant decrease in moist heat resistance from initial starting levels was found for BIs stored at 20 degrees C and either 0 or 20% RH. There was a statistically significant decrease in the B. subtilis BI spore population, compared with initial levels, when the BIs were stored at 20 degrees C and 0% RH concomitant with a significant increase in their EO resistance. BI storage at 20 degrees C and 20 or 44% RH, or under ambient, 4 degrees C, or -20 degrees C conditions, had no significant effect on EO resistance. BIs stored at 20 degrees C and 66% RH demonstrated a significantly lower EO resistance compared with starting levels.