LYMPHOCYTE HOMING AND LEUKOCYTE ROLLING AND MIGRATION ARE IMPAIRED IN L-SELECTIN-DEFICIENT MICE
LYMPHOCYTE HOMING AND LEUKOCYTE ROLLING AND MIGRATION ARE IMPAIRED IN L-SELECTIN-DEFICIENT MICE
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DOI：
10.1016/1074-7613(94)90076-0
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发表时间：
1994-07-01
期刊：
影响因子：
32.4
通讯作者：
TEDDER, TF
中科院分区：
文献类型：
--
作者：
ARBONES, ML;ORD, DC;TEDDER, TF
L-选择素是一种由白细胞表达的细胞粘附分子，介导淋巴细胞与周围淋巴结的高内皮小静脉（HEV）的附着，并介导白细胞与活化的血管内皮之间的最早相互作用。通过基因靶向产生具有突变L-选择素基因的小鼠，该基因导致细胞表面受体表达完全丧失。这些小鼠的淋巴细胞不与外周淋巴结 HEV 结合，并且这些小鼠的外周淋巴结淋巴细胞数量严重减少。短期归巢实验表明，L-选择素还参与淋巴细胞向粘膜淋巴结、派尔氏淋巴结和脾脏的迁移。此外，还观察到响应炎症刺激的白细胞滚动和中性粒细胞迁移到腹膜的显着缺陷。因此; L-选择素在白细胞归巢至淋巴组织和炎症部位中发挥重要作用。
L-selectin, a cell adhesion molecule expressed by leukocytes, mediates the attachment of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes and mediates the earliest interactions between leukocytes and activated vascular endothelium. Mice possessing a mutant L-selectin gene that results in the complete loss of cell surface receptor expression were generated by gene targeting. Lymphocytes from these mice did not bind to peripheral lymph node HEV and these mice had a severe reduction in the number of lymphocytes localized to peripheral lymph nodes. Short-term homing experiments demonstrated that L-selectin was also involved in lymphocyte migration to mucosal lymph nodes, Peyer's patches, and spleen. Furthermore, significant defects in leukocyte rolling and neutrophil migration into the peritoneum in response to an inflammatory stimulus were observed. Thus; L-selectin plays an essential role in leukocyte homing to lymphoid tissues and sites of inflammation.