One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads
One-step detection of pathogens and cancer biomarkers by the naked eye based on aggregation of immunomagnetic beads
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基于免疫磁珠聚集的肉眼一步检测病原体和癌症生物标志物
DOI：
10.1039/c5nr07044a
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发表时间：
2016-01-01
期刊：
影响因子：
6.7
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Chen, Yiping;Xianyu, Yunlei;Jiang, Xingyu
这份报告表明，免疫磁珠除了作为纯化/分离的载体外，还可以作为分析的光学读出器。在外部磁铁的作用下，IMB被吸引到试管的一侧。与靶子特异结合的IMB可以形成一条狭窄的棕色条纹，而游离的IMB将在试管的一侧形成一层弥漫的黄色涂层。目标分析物可以以样品浓度依赖的方式聚集最初分散的IMB，产生肉眼可以看到的从黄色到棕色的颜色变化。该分析结合了侧向流动分析的方便性，允许一步分析在15分钟内完成，以及酶联固相吸附分析的灵敏度。
This report shows that immunomagnetic beads (IMBs) can act as the optical readout for assays, in addition to serving as the carrier for purification/separation. Under the influence of an external magnet, IMBs are attracted to coat one side of a test tube. IMBs specifically bound to targets can form a narrow brown stripe, whereas free IMBs will form a diffuse, yellow coating on the side of the test tube. Target analytes can aggregate initially dispersed IMBs in a sample concentration-dependent manner, yielding a color change from yellow to brown that can be seen with the naked eye. This assay combines the convenience of a lateral flow assay, allowing a one-step assay to finish within 15 min, with the sensitivity of an enzyme-linked immonosorbent assay.