Protein profiles in response to salt stress in leaf sheaths of rice seedlings.
Protein profiles in response to salt stress in leaf sheaths of rice seedlings.
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DOI：
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发表时间：
2005
影响因子：
6.9
通讯作者：
Kanlaya Kong-ngern;S. Daduang;C. Wongkham;S. Bunnag;M. Kosittrakun;P. Theerakulpisut
中科院分区：
文献类型：
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作者：
Kanlaya Kong-ngern;S. Daduang;C. Wongkham;S. Bunnag;M. Kosittrakun;P. Theerakulpisut
盐诱导的泰国水稻品种叶鞘蛋白质合成的变化。采用一维SDS-PAGE和二维PAGE（2DE）对Leaung Anan进行了研究。在SDS-PAGE中，鉴定了在盐胁迫下表达特异性增加的两条蛋白条带（22和31 kDa）。在一年中的不同时间，在温室条件下生长的植物中一致地观察到这些蛋白条带的增强表达。在2DE中，至少有13种不同的蛋白质被诱导，10种不同的蛋白质被盐降低。对耐盐的Pokkali、中度耐盐的Leuang Anan和盐敏感的KDML 105三个品种的水稻幼苗的31 kDa蛋白质进行Western印迹分析，显示出不同的表达模式。与对照植物相比，该蛋白强烈诱导处理的耐盐Pokkali和Leuang Anan叶鞘中的盐。相反，在敏感的KDML 105中，其水平在盐处理下保持不变。此外，水稻cv. Leaung Anan比Pokkali显示出更高的31 kDa蛋白表达。这些结果表明，31 kDa的多肽是一种盐诱导的蛋白质在水稻叶鞘。
Salt-induced changes in protein synthesis in leaf sheaths of the Thai rice cv. Leaung Anan were investigated using one-dimensional SDS-PAGE and two-dimensional PAGE (2DE). In SDS-PAGE, two protein bands (22 and 31 kDa) whose expression was specifically increased under salt stress were identified. Enhanced expression of these protein bands was consistently observed in plants grown under greenhouse conditions saline during different time of the year. In 2DE, at least thirteen different proteins were induced and ten different proteins were decreased by salt. Western blot analysis of the 31 kDa of rice seedlings of three cultivars: salt-tolerant Pokkali, moderately tolerant Leuang Anan, and salt-sensitive KDML 105, showed different expression patterns. The protein was strongly induced by salinity in leaf sheaths of treated tolerant Pokkali and Leuang Anan, compared to control plants. In contrast, its level in the sensitive KDML 105 remained unchanged with salt treatment. Moreover, rice cv. Leaung Anan showed higher expression of the 31 kDa protein than Pokkali. These results suggest that the 31 kDa polypeptide is a salt-induced protein in rice leaf sheaths.