Template-dependent RNA polymerase from black beetle virus-infected Drosophila melanogaster cells.
Template-dependent RNA polymerase from black beetle virus-infected Drosophila melanogaster cells.
复制标题
来自黑甲虫病毒感染的果蝇细胞的模板依赖性 RNA 聚合酶。
DOI：
10.1016/0042-6822(85)90139-4
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Kaesberg,P
中科院分区：
文献类型：
--
作者：
Saunders,K;Kaesberg,P
用黑甲虫病毒（BBV）感染培养的黑腹果蝇细胞，诱导RNA聚合酶与细胞颗粒物质结合，形成一个模板RNA复合物。我们用十二烷基-β-d-麦芽糖苷等洗涤剂处理相关颗粒，使聚合酶溶解。聚合酶的活性依赖于外源RNA，通过用微球菌核酸酶破坏内源模板RNA。添加BBV RNA1或RNA2诱导合成全长负链RNA，与添加的RNA分离成双链复合物。在RNA2复合物中也检测到新合成的正链。某些其他病毒rna也诱导其负链的合成。
Infection of cultured cells ofDrosophila melanogasterwith black beetle virus (BBV) induces an RNA polymerase that is bound to cellular particulate material in a complex with a template RNA. We have solubilized the polymerase by treatment of the relevant particulates with detergents such as dodecyl-β-d-maltoside. The polymerase activity was made dependent upon exogenous RNA by destruction of the endogenous template RNA with micrococcal nuclease. Addition of BBV RNA1 or RNA2 induced synthesis of full-length negative-strand RNA isolated as a double-stranded complex with the added RNA. Newly synthesized plus strands were also detected in the RNA2 complexes. Certain other viral RNAs also induced synthesis of their negative strands.