Effect of passive immunization against inhibin on FSH secretion, folliculogenesis and ovulation rate during the follicular phase of the estrous cycle in mares
Effect of passive immunization against inhibin on FSH secretion, folliculogenesis and ovulation rate during the follicular phase of the estrous cycle in mares
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DOI：
10.1016/s0093-691x(98)00160-5
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发表时间：
1998-09-01
期刊：
影响因子：
2.8
通讯作者：
Taya, K
中科院分区：
文献类型：
--
作者：
Nambo, Y;Kaneko, H;Taya, K
通过使用在去势山羊中产生的抑制素抗血清进行被动免疫，研究了抑制素在配偶中的生理作用。排卵后第 8 天单次肌内注射 PGF2α 后 4 天，对 8 匹母马静脉注射 100 mL（n=4）或 200 mL（n=4）抑制素抗血清，4 匹对照母马以相同方式用 100 mt 去势山羊血清治疗。用血清处理后收集颈静脉血样直至处理后192小时。每隔 24 小时通过超声检查监测卵泡生长和排卵。使用大鼠垂体细胞培养系统在体外检查了抑制素抗血清中和马抑制素生物活性的能力。通过添加增加剂量的抑制素抗血清，可以逆转马卵泡液对培养的大鼠垂体细胞分泌FSH的抑制，从而表明其生物活性。接受抑制素抗血清处理的母马的血浆 FSH 和雌二醇 17 β 水平较高。接受抗血清处理的母马（100 mL = 3.75 +/- 0.63；200 mL = 4.50 +/- 0.65）的排卵率显着高于对照母马（1.25 +/- 0.25）。这些结果表明，抑制素对于调节母马 FSH 分泌和卵泡发生很重要。他们还表明，中和抑制素的生物活性可能成为控制雄性卵泡发生和排卵率的新方法。 (C) 1998 年，爱思唯尔科学公司。
Physiological roles of inhibin in mates were investigated by means of passive immunization using an antiserum to inhibin that had been raised in a castrated goat. Eight mares were given an intravenous injection of either 100 mL (n=4) or 200 mL (n=4) of inhibin antiserum 4 d after a single intramuscular injection of PGF2alpha on Day 8 after ovulation, 4 control mares were treated with 100 mt castrated goat serum in the same manner. Jugular vein blood samples were collected after treatment with the serum until 192 h post treatment. Follicular growth and ovulations were monitored by ultrasound examination at 24-h intervals. The ability of the inhibin antiserum to neutralize the bioactivity of equine inhibin was examined in vitro using a rat pituitary cell culture system.Suppression of secretion of FSH from cultured rat pituitary cells by equine follicular fluid was reversed by the addition of increasing doses of the inhibin antiserum, thereby indicating its bioactivity. Plasma levels of FSH and estradiol-17 beta were higher in mares treated with the inhibin antiserum. The ovulation rate was significantly higher in mates treated with antiserum (100 mL = 3.75 +/- 0.63; 200 mL = 4.50 +/- 0.65) than in control mares (1.25 +/- 0.25). These results demonstrate that inhibin is important in regulating FSH secretion and folliculogenesis in mares. They also show that neutralization of the bioactivity of inhibin may become a new method for the control of folliculogenesis and ovulation rate in mates. (C) 1998 by Elsevier Science Inc.