A diet high in saturated fat and sucrose alters glucoregulation and induces aortic fatty streaks in New Zealand White rabbits.
A diet high in saturated fat and sucrose alters glucoregulation and induces aortic fatty streaks in New Zealand White rabbits.
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DOI：
10.1080/15604280214274
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发表时间：
2002-07
期刊：
影响因子：
--
通讯作者：
Yang Y
中科院分区：
文献类型：
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作者：
Yin W;Yuan Z;Wang Z;Yang B;Yang Y
本研究建立了一种新的、方便的动物模型，用于研究糖尿病周围血管和冠状动脉疾病。体重约 2 kg 的雄性新西兰白兔被分为 2 组：正常对照组喂养标准实验室饲料，糖尿病饮食喂养组接受高脂肪/高蔗糖饮食。高脂/高蔗糖饲料（含10%猪油和37%蔗糖）喂养维持6个月。每月或每两个月对血浆总胆固醇、高密度脂蛋白（HDL）胆固醇、甘油三酯、超氧化物歧化酶、一氧化氮、一氧化氮合酶、胰岛素和葡萄糖进行定量。用苏丹 IV 进行脂质染色后，对主动脉脂肪条纹病变进行量化。通过电子显微镜观察主动脉样品。诱导高血浆甘油三酯和葡萄糖浓度。 6个月结束时，动物血管标本中出现主动脉脂肪条纹病变。据我们所知，这是第一份证明新西兰白兔通过喂养高脂肪/高蔗糖饮食会出现明显主动脉脂肪纹的报告。我们的结果表明，喂食高脂肪/高蔗糖饮食的新西兰白兔将为研究糖尿病周围血管和冠状动脉疾病提供一个方便的模型。
A new and convenient animal model for studying peripheral vascular and coronary artery disease in diabetes was established in this study. Male New Zealand White rabbits weighing approximately 2 kg were divided into 2 groups: a normal control group fed standard laboratory chow and a diabetogenic diet–fed group received a high-fat/high-sucrose diet. The high-fat/high-sucrose diet (contained 10% lard and 37% sucrose) feeding was maintained for 6 months. Plasma total cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride, superoxide dismutase, nitric oxide, nitric oxide synthase, insulin, and glucose were quantitated at monthly or bimonthly intervals. The aortic fatty streak lesions were quantified following lipid staining with Sudan IV. The aortic samples were observed by electron microscopy. High plasma triglyceride and glucose concentrations were induced. At the end of 6 months, the aortic fatty streak lesions were present in the animals' vascular specimens. As far as we know, this is the first report that demonstrates that New Zealand White rabbits can develop obvious aortic fatty streaks by feeding a high-fat/high-sucrose diet. Our results suggest that NewZealand White rabbits fed a high-fat/high-sucrose diet would provide a convenient model for studying peripheral vascular and coronary artery disease in diabetes.