Depletion of donor lymphocytes by counterflow centrifugation successfully prevents acute graft-versus-host disease in matched allogeneic marrow transplantation.
Depletion of donor lymphocytes by counterflow centrifugation successfully prevents acute graft-versus-host disease in matched allogeneic marrow transplantation.
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通过逆流离心去除供体淋巴细胞可成功预防匹配同种异体骨髓移植中的急性移植物抗宿主病。
DOI：
10.1182/blood.v67.5.1302.1302
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发表时间：
1986
期刊：
影响因子：
20.3
通讯作者：
C. Haanen
中科院分区：
文献类型：
--
作者：
T. D. Witte;J. Hoogenhout;B. Pauw;R. Holdrinet;Jacques J.M. Janssen;J. Wessels;W. V. Daal;T. Hustinx;C. Haanen
采用密度浮选离心和逆流洗脱相结合的方法，将22例组织相容的兄弟姐妹骨髓中98%的淋巴细胞去除。即使有甲氨蝶呤（MTX）的骨髓抑制作用，造血干细胞的活力也没有受到影响，这是可评估患者移植后正常再生所表明的。一名患者（UPN 9）表现出原发性移植物衰竭，可能是由于持续败血症和长期抗生素治疗。2例患者有持续性宿主淋巴细胞，其中1例在复发时检查；另一个仍处于缓解期。2例患者在骨髓移植（BMT）后未接受免疫抑制，两例患者均出现急性移植物-v-宿主病（GVHD）。9例患者在BMT后接受MTX作为免疫抑制。所有患者均未发生GVHD，但有5名患者在移植后立即发生了致命感染。11例患者移植后接受环孢素治疗。从BMT后第5周开始，CsA逐渐被MTX替代。这些患者均未发生急性GVHD、严重慢性GVHD或致死性感染。通过逆流离心去除98%的淋巴细胞可以防止急性GVHD的发展，前提是在BMT后给予免疫抑制。没有观察到移植排斥反应，但目前可评估的患者数量有限。
Bone marrow from 22 histocompatible siblings was depleted of 98% of the lymphocytes using a combination of density flotation centrifugation followed by counterflow elutriation. Even with the marrow suppressive influence of methotrexate (MTX), the viability of the hematopoietic stem cells was not affected, as indicated by the normal repopulation after grafting in the evaluable patients. One patient (UPN 9) showed a primary graft failure, possibly resulting from persisting septicemia and long-term antibiotic therapy. Two patients have persistent host lymphocytes, one of whom was examined during relapse; the other remains in remission. Two patients did not receive immunosuppression after bone marrow transplantation (BMT), and acute graft-v-host disease (GVHD) developed in both. Nine patients received MTX as immunosuppression following BMT. GVHD did not develop in any of them, but fatal infections in the immediate posttransplant period developed in five patients. Eleven patients received cyclosporine (CsA) after transplantation. Beginning in week 5 after BMT, CsA was gradually replaced by MTX. Acute GVHD, substantial chronic GVHD, or fatal infections did not develop in any of these patients. Removal of 98% of the lymphocytes by counterflow centrifugation prevents development of acute GVHD, provided that immunosuppression is administered after BMT. Graft rejection was not observed, but the number of evaluable patients is limited at present.
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影响因子：
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影响因子：
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作者：
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