Insulin promotes electrical coupling between cultured sympathetic neurons-猫眼课题宝

胰岛素促进培养的交感神经元之间的电耦合

DOI：

10.1523/jneurosci.05-07-01675.1985

发表时间：

1985

期刊：

Neuropeptides

影响因子：

2.9

通讯作者：

中科院分区：

医学3区

文献类型：

作者：

E. Wolinsky;P. Patterson;A. Willard

将神经元置于组织培养中是研究环境因素如何影响其分化的一种方法。血清的替换--用确定的成分补充培养基扩展了实验者对培养环境的控制;然而，它也引入了额外的潜在影响。在这份报告中，我们证实了希金斯和伯顿的观察（希金斯，D.，和H. Burton（1982）Neuroscience 7：2241-2253），与大鼠交感神经元的血清补充培养物相比，无血清培养物中的电耦合频率增加。此外，还进行了实验，以确定这种影响是由于去除血清还是由于向培养环境中添加成分确定的培养基组分所致。测试适用于交感神经元的确定成分培养基配方的各个成分的结果（Botanical，J.E.，和G. H. Sato（1979）Proc. Natl. Acad. Sci.联合S. A. 76：514-517）显示胰岛素能够在无血清培养物中诱导电偶联。因此，交感神经元的电突触的形成可以被神经调控。

Placing neurons in tissue culture is one way to study how environmental factors affect their differentiation. Replacement of serum- supplementation of the culture medium with defined ingredients extends the experimenter's control of the culture environment; however it also introduces additional potential influences. In this report, we confirm the observation of Higgins and Burton (Higgins, D., and H. Burton (1982) Neuroscience 7:2241–2253) of increased frequency of electrical coupling in serum-free compared to serum-supplemented cultures of rat sympathetic neurons. In addition, experiments were performed to determine whether this effect results from the removal of serum or from the addition of the defined medium components to the culture environment. The results of testing individual ingredients of the defined medium recipe adapted for use on sympathetic neurons (Bottenstein, J.E., and G. H. Sato (1979) Proc. Natl. Acad. Sci. U. S. A. 76:514–517) show that insulin is capable of inducing electrical coupling in serum-free cultures. Thus, the formation of electrical synapses by sympathetic neurons can be hormonally regulated.

Determination of synaptic phenotype: insulin and cAMP independently initiate development of electrotonic coupling between cultured sympathetic neurons.