The repetitive domain of Trypanosoma cruzi trans-sialidase enhances the immune response against the catalytic domain
The repetitive domain of Trypanosoma cruzi trans-sialidase enhances the immune response against the catalytic domain
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DOI：
10.1086/514199
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发表时间：
1998-02-01
影响因子：
6.4
通讯作者：
Frasch, ACC
中科院分区：
文献类型：
--
作者：
Buscaglia, CA;Campetella, O;Frasch, ACC
克氏锥虫转唾液酸酶由基本上由免疫显性氨基酸重复单元（SAA-重复）组成的C-末端结构域和负责酶活性的氨基区域（催化结构域）组成。为了研究SAA重复序列的可能功能，在小鼠中测试了含有或缺乏C-末端区域的重组转唾液酸酶。静脉注射的蛋白质中存在的SAP重复序列有两个后果。首先，它们增强了血液中转唾液酸酶活性的持久性。其次，SAP重复序列促进了针对抑制转唾液酸酶活性的催化结构域的抗体的产生。这些结果表明，SAP重复调节血液中的转唾液酸酶活性。
Trypanosoma cruzi trans-sialidase consists of a C-terminal domain composed essentially of immunodominant amino acid repeat units (SAPA-repeats) and an amino region responsible for the enzymatic activity (catalytic domain). To investigate the possible function(s) of SAPA-repeats, recombinant trans-sialidases either containing or lacking the C-terminal region were tested in mice. The presence of SAPA-repeats in the intravenously injected protein has two consequences. First, they enhance the persistence of the trans-sialidase activity in blood. Second, SAPA-repeats promoted the production of antibodies directed to the catalytic domain that inhibit trans-sialidase activity. These results suggest that SAPA-repeats modulate the trans-sialidase activity in blood.