Evolution and mutations of hepatitis B virus quasispecies in genotype B and C during vertical transmission
Evolution and mutations of hepatitis B virus quasispecies in genotype B and C during vertical transmission
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B型和C型乙型肝炎病毒准种在垂直传播过程中的进化和突变
DOI：
10.1002/jmv.24424
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发表时间：
2016-06-01
影响因子：
12.7
通讯作者：
Wang, Yuming
中科院分区：
文献类型：
--
作者：
Wu, Quanxin;Xu, Cheng;Wang, Yuming
垂直传播过程中基因型B和C之间HBV QS的演变模式尚不清楚。在这项研究中，我们招募了10对HBV感染的母婴对（4对基因型B，4对基因型C，2对合并感染），没有抗病毒治疗。对母婴血清HBV DNA进行测序，分析HBV QS复杂度和多样性，记录多态性和突变位点，并进行系统进化树分析。结果显示，C基因型组母亲的P（氨基酸）、C/PreC（氨基酸）和PreS 1（核苷酸）基因的QS复杂度显著高于婴儿（P0.05）。与C基因型不同，婴儿P基因的QS复杂度（核苷酸）显著高于母亲（P0.05）。B基因型的全长和大部分基因的QS差异在母婴间无显著性差异（P>0.05），与C基因型配对，P、X、RT基因的dS、Pre S1基因（氨基酸）的遗传距离和Pre S1基因的dN在母婴间差异显著（P
Evolution patterns of HBV QS between genotype B and C during vertical transmission are not well understood. In this study, we enrolled 10 HBV infected mother-infant pairs (four pairs with genotype B, four pairs with genotype C, and two with co-infection) without anti-viral therapy. Serum HBV DNA of mothers and infants were sequenced, HBV QS complexity and diversity were analyzed, polymorphisms and mutation sites were recorded, and phylogenetic trees were performed. Our result showed that the QS complexities in P (amino acid), C/PreC (amino acid), and PreS1 (nucleotide) gene were significantly higher in mothers than in infants in pairs with genotype C (P0.05). Unlike genotype C, QS complexity of P gene (nucleotide) was significantly higher in infants than in mothers (P0.05). QS diversities of full-length and most genes in genotype B were comparable between mothers and their infants (P>0.05), in pairs with genotype C, dS of P, X, RT genes, genetic distance of Pre S1 gene (amino acid) and dN of Pre S1 gene were significant higher in mothers than in infants (P