Immunohistochemical detection of advanced glycation end products in human bladder with specific monoclonal antibody
Immunohistochemical detection of advanced glycation end products in human bladder with specific monoclonal antibody
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DOI：
10.1111/j.1442-2042.2009.02259.x
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发表时间：
2009-04-01
影响因子：
2.6
通讯作者：
Yoshida, Masaki
中科院分区：
文献类型：
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作者：
Matsumoto, Kenshi;Fujiwara, Yukio;Yoshida, Masaki
目的：探讨晚期糖基化终末产物（AGE）在人膀胱中的积累情况。方法：取9例根治性膀胱切除术患者的膀胱标本。通过三种不同的单克隆抗 AGE 抗体（例如抗 N(ε)-(羧甲基)赖氨酸 (CML)、抗咪唑酮和抗戊糖素抗体）对冰冻切片进行免疫组织化学分析。通过间接免疫过氧化物酶方法用这些抗体对膀胱切片进行染色。还使用一种抗 AGE 抗体或抗人巨噬细胞抗体进行了双重免疫组织化学染色。结果：我们证明 CML 和戊糖苷在人膀胱细胞外和细胞内积累，而没有观察到咪唑啉酮的任何积累。双重免疫组化染色表明，人膀胱中AGE积累的细胞来源于巨噬细胞。结论：本研究表明，AGE结构如CML和戊糖苷在人膀胱细胞外积累，并被组织巨噬细胞内吞。
Objective: To investigate the accumulation of advanced glycation end products (AGE) in human bladder.Methods: Human bladder specimens were obtained from nine patients during radical cystectomy. Frozen sections were immunohistochemically analyzed by three different monoclonal anti-AGE antibodies such as anti-N(epsilon)-(carboxymethyl)lysine (CML), anti-imidazolone and anti-pentosidine antibodies. Bladder sections were stained with these antibodies by indirect immunoperoxidase methods. Double immunohistochemical staining with one of the anti-AGE antibodies or an anti-human macrophage antibody was also carried out.Results: We demonstrated that CML and pentosidine were accumulated in human bladder extracellularly as well as intracellularly, whereas any accumulation of imidazolone was not observed. Double immunohistochemical staining indicated that AGE-accumulated cells in human bladder were derived from macrophages.Conclusions: The present study demonstrated that AGE-structures such as CML and pentosidine are accumulated extracellularly in human bladder, and were endocytosed by tissue macrophages.