Phloroglucinol (PG) purified from Ecklonia cava attenuates radiation-induced apoptosis in blood lymphocytes and splenocytes
Phloroglucinol (PG) purified from Ecklonia cava attenuates radiation-induced apoptosis in blood lymphocytes and splenocytes
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DOI：
10.1016/j.fct.2011.06.021
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发表时间：
2011-09-01
影响因子：
4.3
通讯作者：
Jee, Youngheun
中科院分区：
文献类型：
--
作者：
Park, Suk-Jae;Ahn, Ginnae;Jee, Youngheun
间苯三酚（PG），一种多酚化合物的Ecklonia cava被称为褐藻丰富的济州岛，已被提出发挥抗氧化和细胞保护作用对氧化应激。本研究证实了PG对电离辐射损伤小鼠的保护作用，并对其保护机制进行了详细的研究。结果表明，PG能提高脾细胞活力，无细胞毒性。PG通过抑制2Gy照射脾细胞产生活性氧，抑制亚G（1）DNA含量的增加，从而显著促进脾细胞的增殖。此外，PG显着降低DNA损伤和凋亡片段的数量在淋巴细胞对氧化应激。also.与仅电离辐射照射的小鼠相比，PG增加了内源性脾CFU的计数。这些结果表明PG在小鼠中对电离辐射引起的氧化应激的多方面保护机制，提供抑制细胞凋亡和增强造血的益处。皇冠版权所有（C）2011由爱思唯尔有限公司出版。保留所有权利。
Phloroglucinol (PG), a polyphenol compound of Ecklonia cava known as brown algae abundant in Jeju island, has been proposed to exert the antioxidative and cytoprotective effects against oxidative stress. In this study, we confirmed that PG protected mice from damages caused by ionizing radiation and investigated its protection mechanism in detail. The result showed that PG increased the viability of splenocytes without cytotoxicity. Also, PG significantly enhanced the proliferation of splenocytes by limiting the increment of sub-G(1) DNA contents via the inhibition of reactive oxygen species production in 2 Gy-irradiated splenocytes. In addition, PG significantly decreased DNA damage and the number of apoptotic fragments in lymphocytes against oxidative stress. Also. PG increased the counts of endogenous spleen CFUs, compared with only ionizing radiation-irradiated mice. These results demonstrate the multi-faceted protection mechanisms of PG in mice against oxidative stress caused by ionizing radiation, providing the benefit of inhibiting apoptosis and strengthening hematopoiesis. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.