Growth and differentiation of human esophageal carcinoma cell lines.
Growth and differentiation of human esophageal carcinoma cell lines.
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发表时间：
1986
期刊：
影响因子：
11.2
通讯作者：
S. Banks-Schlegel;J. Quintero
中科院分区：
文献类型：
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作者：
S. Banks-Schlegel;J. Quintero
人食管癌细胞系（8个细胞系）在形态外观、生长特性和某些分化功能（即角蛋白和交联包膜）的表达方面与正常细胞系不同。与正常人食管角质形成细胞相反，癌细胞是多形性的，并且倾向于以无组织的方式堆积。当在最佳生长条件下生长时，癌细胞通常会比未转化的细胞生长到更高的饱和密度；他们的世代时间是可变的。转化的细胞在严格的生长条件下（例如，减少血清并且除了氢化可的松之外没有其他生长因子）比未转化的人食管角质形成细胞生长得更好，但它们的生长在这些条件下仍然受到限制。当以克隆密度（5 X 10(3) 细胞/60-mm 培养皿）生长时，癌细胞保留对 3T3 饲养层的要求，但如果以高于克隆密度（10(5) 细胞/60-mm 培养皿）铺板，则可以在没有饲养层的情况下传代和维持。尽管不同细胞系的集落形成效率和集落大小各不相同，但所有细胞系在软琼脂糖中均以不依赖贴壁的方式生长。并非所有贴壁依赖性细胞系都具有致瘤性。通过使用源自软琼脂糖选择的克隆的细胞系，大大增强了致瘤潜力。在癌细胞系中观察到角蛋白和交联包膜表达的改变，并且通常反映了在原发性食管癌中观察到的那些变化。在两种细胞系（HCE-4 和 HCE-6）中，Mr 44,000（类似于 Rheinwald 的 Mr 40,000 角蛋白）和 52,000 角蛋白的合成受到抑制，与源自这些细胞系的肿瘤中 67 Kd 角蛋白的出现一致。这些角蛋白模式在从这些肿瘤重新培养的细胞系中再次逆转，表明这些特定角蛋白的表达受到外在生长调节。角质形成细胞终末分化的另一个特征，即交联包膜形成，被发现在大多数但不是全部人食管癌细胞系中显着改变（减少）。
Human esophageal carcinoma cell lines (8 cell lines) differed from their normal counterpart in terms of their morphological appearance, growth properties, and the expression of certain differentiated functions, namely keratin proteins and cross-linked envelopes. In contrast to normal human esophageal keratinocytes, the carcinoma cells were pleomorphic and tended to pile up in an unorganized fashion. When grown under optimal growth conditions the carcinoma cells generally grew to a higher saturation density than their nontransformed counterpart; their generation times were variable. Transformed cells grew better under stringent growth conditions (e.g., decreased serum and no additional growth factors except hydrocortisone) than did nontransformed human esophageal keratinocytes but their growth was still restricted under these conditions. The carcinoma cells retained a requirement for a 3T3 feeder layer when grown at clonal densities (5 X 10(3) cells/60-mm dish) but could be passaged and maintained without a feeder layer if plated at higher than clonal densities (10(5) cells/60-mm dish). All cell lines grew in an anchorage-independent fashion in soft agarose although the colony forming efficiency and size of the colonies varied among the different cell lines. Not all anchorage-independent cell lines were tumorigenic. Tumorigenic potential was greatly augmented by the use of cell lines derived from soft agarose selected clones. Altered expression of keratin proteins and cross-linked envelopes was observed in the carcinoma cell lines and generally reflected those changes seen in primary esophageal carcinomas. In two cell lines (HCE-4 and HCE-6), the synthesis of the Mr 44,000 (analogous to Rheinwald's Mr 40,000 keratin) and 52,000 keratins was suppressed coincident with the appearance of the 67 Kd keratin in tumors derived from these cell lines. These keratin patterns were once again reversed in cell lines recultured from these tumors, suggesting that the expression of these specific keratins is subject to extrinsic growth regulation. Another feature of terminal differentiation in keratinocytes, cross-linked envelope formation, was found to be significantly altered (reduced) in most but not all human esophageal carcinoma cell lines.