Uptake of latex particles by pulmonary macrophages: role of calcium.
Uptake of latex particles by pulmonary macrophages: role of calcium.
复制标题
肺巨噬细胞摄取乳胶颗粒：钙的作用。
DOI：
10.1152/ajpcell.1983.245.3.c227
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Brain,JD
中科院分区：
文献类型：
--
作者：
Parod,RJ;Brain,JD
活的和固定的仓鼠肺巨噬细胞对乳胶颗粒的摄取是钙和胰蛋白酶敏感的。颗粒摄取没有刺激45 Ca的摄取。然而，当用A23187刺激45 Ca摄取时，颗粒摄取被抑制。当钴与A23187一起加入时，~（45）Ca的摄取被抑制，颗粒摄取恢复到对照水平。A23187、细胞松弛素B和A23187加细胞松弛素B均以相同程度降低颗粒摄取。虽然A23187和哇巴因在细胞内Na+和K+水平产生类似的变化，但只有A23187抑制颗粒摄取。我们的结论是，细胞外Ca 2+通过其与肺巨噬细胞膜中的胰蛋白酶敏感受体的相互作用，促进颗粒细胞结合。相反，升高的细胞内Ca 2+水平抑制颗粒摄入，但不附着。
The uptake of latex particles by both viable and fixed hamster pulmonary macrophages was calcium and trypsin sensitive. Particle uptake did not stimulate the uptake of 45Ca. However, when 45Ca uptake was stimulated with A23187, particle uptake was inhibited. When cobalt was added with A23187, the uptake of 45Ca was inhibited and particle uptake returned to control levels. A23187, cytochalasin B, and A23187 plus cytochalasin B all reduced particle uptake to the same extent. Although both A23187 and ouabain produced similar changes in the intracellular levels of Na+ and K+, only A23187 inhibited particle uptake. We conclude that extracellular Ca2+ promotes particle-cell binding through its interaction with a trypsin-sensitive receptor in the pulmonary macrophage membrane. In contrast, elevated intracellular Ca2+ levels inhibit particle ingestion but not attachment.