ANALYSIS OF THE GENETIC-POLYMORPHISM OF COAGULATION FACTOR-XIIIB (FXIIIB) BY ISOELECTRIC-FOCUSING
ANALYSIS OF THE GENETIC-POLYMORPHISM OF COAGULATION FACTOR-XIIIB (FXIIIB) BY ISOELECTRIC-FOCUSING
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DOI：
10.1002/elps.1150090814
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发表时间：
1988-08-01
期刊：
影响因子：
2.9
通讯作者：
CLEVE, H
中科院分区：
文献类型：
--
作者：
LEIFHEIT, HJ;CLEVE, H
凝血因子XIIIB（FXIIIB）的遗传变异体通过等电聚焦进行分析，在琼脂糖凝胶中进行，然后进行免疫固定。FXIIIB表型通过使用考马斯亮蓝R-250和硝酸银银的组合染色程序可视化。在聚丙烯酰胺凝胶中，通过在补充有载体两性电解质的固定化pH梯度中进行混合等电聚焦，实现了分辨率的提高。我们检查了来自德国南部的1604名无关的健康个体。FXIIIB等位基因的频率为B*1 = 0.7581、B*2 = 0.0843、B*3 = 0.1568和B*4 - 0.0019。亲子关系争议的理论排除率为22.35%。
The genetic variants of the coagulation factor XIIIB (FXIIIB) were analyzed by isoelectric focusing, carried out in agarose gels and followed by immunofixation. The FXIIIB phenotypes were visualized by a combined staining procedure with Coomassive Brilliant Blue R-250 and silver nitrate. Improved resolution was accomplished in polyacrylamide gels by hybrid isoelectric focusing in immobilized pH gradients supplemented with carrier ampholytes. We examined a total of 1.604 unrelated, healthy individuals from Southern Germany. The frequencies for the FXIIIB alleles were B*1 = 0.7581, B*2 = 0.0843, B*3 = 0.1568 and B*4 - 0.0019. The theoretical exclusion rate for disputed paternity is 22.35%.