Measures of Bone Mineral Carbonate Content and Mineral Maturity/Crystallinity for FT-IR and Raman Spectroscopic Imaging Differentially Relate to Physical–Chemical Properties of Carbonate-Substituted Hydroxyapatite
Measures of Bone Mineral Carbonate Content and Mineral Maturity/Crystallinity for FT-IR and Raman Spectroscopic Imaging Differentially Relate to Physical–Chemical Properties of Carbonate-Substituted Hydroxyapatite
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DOI：
10.1007/s00223-021-00825-4
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发表时间：
2021-03
影响因子：
4.2
通讯作者：
Erik A. Taylor;C. Mileti;Sandhya Ganesan;Joo Ho Kim;E. Donnelly
中科院分区：
文献类型：
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作者：
Erik A. Taylor;C. Mileti;Sandhya Ganesan;Joo Ho Kim;E. Donnelly
由振动光谱学评估的骨矿物碳酸盐含量与骨折发生率有关，而矿物成熟度/结晶度(MMC)与组织年龄有关。随着FT-IR和拉曼光谱在临床前研究和翻译研究中越来越广泛地用于表征骨的化学成分，它们的骨矿物结果需要改进的验证，以提供光谱数据的解释。在这项研究中，我们的目标是(1)将拉曼和FT-IR碳酸盐：磷酸盐比率通过直接积分峰与金标准碳酸盐含量和基本亚带比率的分析测量来计算；(2)将拉曼和FT-IR MMC测量与化学标准和天然骨粉中的晶体尺寸的金标准分析测量相关联。随着碳酸盐含量的增加(拉曼：P <； 0.0 1，R2= 0.87；FT-IR：p <； 0.0 1，R2= 0.96)，拉曼和FT-IR直接积分碳酸盐/磷酸盐比值增加，拉曼对碳酸盐含量比FT-IR更敏感(拉曼斜率 + 95%vs FT-IR斜率，p <； 0.0 1)。拉曼光谱和傅立叶变换红外光谱的MMC均随晶体尺寸增大而增大(拉曼：p <； 0.01，R2= 0.76；FT-IR p <； 0.01，R2= 0.73)，且FT-IR比拉曼更敏感(c轴长度：斜率FT-IR MMC + 111%vs拉曼MMC，p <； 0.01)。此外，FT-IR而不是拉曼光谱检测到碳酸化羟基磷灰石(CHA)和结晶度较差的羟基磷灰石(HA)的MMC与晶粒度的关系存在差异(斜率CHA + 87%vs HA，p <； 0.01)。综合起来，这些结果有助于未来的研究能够阐明碳酸盐含量与骨折之间的关系，并提供对天然骨矿物的FT-IR和拉曼光谱的优势和局限性的洞察。
Bone mineral carbonate content assessed by vibrational spectroscopy relates to fracture incidence, and mineral maturity/ crystallinity (MMC) relates to tissue age. As FT-IR and Raman spectroscopy become more widely used to characterize the chemical composition of bone in pre-clinical and translational studies, their bone mineral outcomes require improved validation to inform interpretation of spectroscopic data. In this study, our objectives were (1) to relate Raman and FT-IR carbonate:phosphate ratios calculated through direct integration of peaks to gold-standard analytical measures of carbonate content and underlying subband ratios; (2) to relate Raman and FT-IR MMC measures to gold-standard analytical measures of crystal size in chemical standards and native bone powders. Raman and FT-IR direct integration carbonate:phosphate ratios increased with carbonate content (Raman: p < 0.01, R2= 0.87; FT-IR: p < 0.01, R2= 0.96) and Raman was more sensitive to carbonate content than the FT-IR (Raman slope + 95% vs FT-IR slope, p < 0.01). MMC increased with crystal size for both Raman and FT-IR (Raman: p < 0.01, R2= 0.76; FT-IR p < 0.01, R2= 0.73) and FT-IR was more sensitive to crystal size than Raman (c-axis length: slope FT-IR MMC + 111% vs Raman MMC, p < 0.01). Additionally, FT-IR but not Raman spectroscopy detected differences in the relationship between MMC and crystal size of carbonated hydroxyapatite (CHA) vs poorly crystalline hydroxyapatites (HA) (slope CHA + 87% vs HA, p < 0.01). Combined, these results contribute to the ability of future studies to elucidate the relationships between carbonate content and fracture and provide insight to the strengths and limitations of FT-IR and Raman spectroscopy of native bone mineral.