Immunoglobulin secreting cells in normal human bronchial lavage fluids.
Immunoglobulin secreting cells in normal human bronchial lavage fluids.
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正常人支气管灌洗液中的免疫球蛋白分泌细胞。
DOI：
10.1172/jci109195
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
P. Stevens
中科院分区：
文献类型：
--
作者：
E. C. Lawrence;R. Blaese;R. Martin;P. Stevens
用反向溶血空斑法对12名正常志愿者支气管灌洗液中的免疫球蛋白分泌细胞进行定量，并与外周血中的免疫球蛋白分泌细胞进行比较。支气管灌洗液中分泌免疫球蛋白（IG）G的细胞数平均为489个/百万淋巴细胞，外周血中分泌IgG的细胞数平均为175个/百万淋巴细胞（P < 0.02）。支气管灌洗液中伊加分泌细胞平均数为633个/百万淋巴细胞，外周血中IgA分泌细胞平均数为100个/百万淋巴细胞（P < 0.005）。因此，与外周血相比，来自灌洗液的细胞相对富集IgG和伊加分泌细胞。然而，伊加分泌细胞是支气管灌洗液中免疫球蛋白分泌细胞的主要类别，而IgG分泌细胞在外周血中占主导地位。支气管灌洗液中IgA/IgG分泌细胞的平均比值为1.26，而外周血中IgA/IgG分泌细胞的平均比值为0.57（P < 0.02）。分泌IgM的细胞仅在研究的7个支气管灌洗液样品中的4个中被鉴定，但在所有外周血样品中。IgE分泌细胞不存在于正常外周血中，但可以证明在11个灌洗液标本中的5。因此，可以在正常人受试者的下支气管肺泡树中鉴定出活跃分泌免疫球蛋白的细胞。分泌IgG、伊加或IgM的细胞可能在局部肺防御感染中起作用;分泌IgE的细胞可能导致肺中的超敏反应。
Immunoglobulin secreting cells were quantitated in the bronchial lavage fluids of 12 normal volunteers and compared with immunoglobulin secreting cells in peripheral blood, by a reverse hemolytic plaque assay. The mean number of cells secreting immunoglobulin (Ig)G in bronchial lavage fluids was 489 per million lymphocytes vs. a mean of 175 IgG secreting cells per million lymphocytes in peripheral blood (P < 0.02). The mean number of IgA secreting cells in bronchial lavage fluids was 633 per million lymphocytes as compared to 100 per million lymphocytes in peripheral blood (P < 0.005). Thus, compared to peripheral blood, cells from the lavage fluids were relatively enriched for both IgG and IgA secreting cells. However, IgA secreting cells were the major class of immunoglobulin secreting cells in bronchial lavage fluids, whereas IgG secreting cells predominated in peripheral blood. The prominence of IgA secreting cells in bronchial lavage fluids was further demonstrated by a mean ratio of IgA/IgG secreting cells in bronchial lavage fluids of 1.26 compared to a ratio in peripheral blood of 0.57 (P < 0.02). Cells secreting IgM were identified in only four of seven bronchial lavage fluid samples studied but in all peripheral blood samples. IgE secreting cells were not present in normal peripheral blood but could be demonstrated in 5 of 11 lavage fluid specimens. Thus, cells actively secreting immunoglobulins can be identified in the lower bronchial-alveolar tree of normal human subjects. Cells secreting IgG, IgA, or IgM may function in local lung defenses against infection; cells secreting IgE may contribute to hypersensitivity reactions in the lung.