A cold responsive ethylene responsive factor from Medicago falcata confers cold tolerance by up-regulation of polyamine turnover, antioxidant protection, and proline accumulation
A cold responsive ethylene responsive factor from Medicago falcata confers cold tolerance by up-regulation of polyamine turnover, antioxidant protection, and proline accumulation
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来自苜蓿的冷响应乙烯响应因子通过上调多胺周转、抗氧化保护和脯氨酸积累来赋予冷耐受性
DOI：
10.1111/pce.13114
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Guo Zhenfei
中科院分区：
文献类型：
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作者：
Zhuo Chunliu;Liang Lu;Zhao Yaqing;Lu Shaoyun;Guo Zhenfei
乙烯应答因子（ERF）亚家族转录因子在植物的非生物和生物胁迫耐受中起着重要作用。MfERF 1是从重要的耐寒豆科牧草苜蓿中分离到的一个冷响应ERF。MfERF 1的过表达导致转基因烟草植物对冷冻和低温的耐受性增加，而苜蓿中MfERF 1的直系同源物的下调导致RNAi植物的冷冻耐受性降低。与野生型相比，转基因植株中一些胁迫响应基因（CHN 50、OSM、ERD 10 C和SAMS）、与亚精胺（Spd）和精胺（Spm）合成有关的基因（SAMDC 1、SAMDC 2、SPDS 1、SPDS 2和SPMS）和catenase（PAO）的转录水平明显提高。然而，无论是Spd或Spm水平积累在转基因植株中作为一个结果，促进多胺氧化酶的活性。与野生型相比，转基因植株具有更高的抗氧化剂活性，这与诱导的编码基因（包括Cu、Zn-SOD、CAT 1、CAT 2、CAT 3和cpAP X）相关，并且与诱导的P5 CS相关的脯氨酸积累更多，PROX 2转录减少。结果表明，MfERF 1通过促进多胺周转、抗氧化保护和脯氨酸积累来增强抗寒性。
Ethylene responsive factor (ERF) subfamily transcription factors play an important role in plant abiotic and biotic stress tolerance. A cold responsive ERF,MfERF1, was isolated fromMedicago falcata, an important forage legume that has great cold tolerance. Overexpression ofMfERF1resulted in an increased tolerance to freezing and chilling in transgenic tobacco plants, whereas down‐regulation of the ortholog ofMfERF1inMedicago truncatularesulted in reduced freezing tolerance in RNAi plants. Higher transcript levels of some stress responsive genes (CHN50,OSM,ERD10C, andSAMS) and those involved in spermidine (Spd) and spermine (Spm) synthesis (SAMDC1,SAMDC2,SPDS1,SPDS2, andSPMS) and catabolism (PAO) were observed in transgenic plants than in wild type. However, neither Spd nor Spm level was accumulated in transgenic plants as a result of promoted polyamine oxidase activity. Transgenic plants had higher activities of antioxidants associated with the induced encoding genes includingCu,Zn‐SOD,CAT1,CAT2,CAT3, andcpAPXand accumulated more proline associated with inducedP5CSand reducedPROX2transcription as compared with wild type. The results suggest thatMfERF1confers cold tolerance through promoted polyamine turnover, antioxidant protection, and proline accumulation.