MAGNETIC MONOSIZED POLYMER PARTICLES FOR FAST AND SPECIFIC FRACTIONATION OF HUMAN MONONUCLEAR-CELLS
MAGNETIC MONOSIZED POLYMER PARTICLES FOR FAST AND SPECIFIC FRACTIONATION OF HUMAN MONONUCLEAR-CELLS
复制标题
DOI：
10.1111/j.1365-3083.1985.tb01873.x
复制
发表时间：
1985-01-01
影响因子：
3.7
通讯作者：
UGELSTAD, J
中科院分区：
文献类型：
--
作者：
LEA, T;VARTDAL, F;UGELSTAD, J
磁性单分散聚合物颗粒的开发和必要的条件建立使用它们的人外周血单核细胞群体的定量和分级。该颗粒由苯乙烯二乙烯基苯核心，磁铁矿已被沉积在原位氧化过程。此后，用含有环氧基和羟基的亲水性聚合物涂覆芯。该颗粒对蛋白质具有强的非特异性结合能力，并且仅通过物理吸附就可以用适当的抗体包被。然而，外部聚合物上的羟基也使得共价偶联成为可能。在适当封闭后，它们可用于玫瑰花结试验，用于定量先前用单克隆抗体致敏的单核白细胞。此外，合适的磁体使得可以有效地耗尽细胞悬浮液中的玫瑰花结形成细胞。我们已经彻底调查了人类单核细胞的功能特性耗尽的T淋巴细胞通过这种技术。我们的研究结果表明，T细胞几乎完全消除，如流式细胞术和各种功能测定系统所示。
Magentic monodisperse polymer particles were developed and the necessary conditions established to use them for both quantification and fractionation of human peripheral blood mononuclear cell populations. The particles consist of a styrene divinylbenzene core into which magnetite has been deposited by an in situ oxidation process. Thereafter the core has been coated with a hydrophilic polymer containing epoxy and hydroxyl groups. The particles have strong nonspecific binding capacity for protein and can be coated with the appropriate antibodies by physical adsorption only. However, the hydroxyl groups on the outer polymer also make covalent coupling possible. After appropriate blocking they can be used in a rosette assay for quantification of mononuclear leukocytes previously sensitized with monoclonal antibodies. Furthermore, a suitable magnet makes it possible to deplete the cell suspension efficiently of the rosette-forming cells. We have thoroughly investigated the functional properties of human mononuclear cells depleted of T lymphocytes by this technique. Our results show that T cells are virtually completely eliminated, as demonstrated by flow cytometry and various functional assay systems.