TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C60) primary ions
TOF-SIMS 3D biomolecular imaging of Xenopus laevis oocytes using buckminsterfullerene (C60) primary ions
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DOI：
10.1021/ac061370u
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发表时间：
2007-03-15
影响因子：
7.4
通讯作者：
Vickerman, John C.
中科院分区：
文献类型：
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作者：
Fletcher, John S.;Lockyer, Nicholas P.;Vickerman, John C.
以富勒烯（C - 60）作为一次离子源的飞行时间二次离子质谱（TOF - SIMS）能够以高灵敏度和极小的化学损伤生成表面的化学图像。我们研究了C - 60（+）以可控方式对生物细胞表面进行深度剖析以及随后对所揭示的亚表面进行成像的应用，以便生成生物系统的三维分子图像。这样一种分析工具不仅能够绘制分子种类的表面定位图，还能够在极少的样品制备/干预下研究生物分子随深度的分布。在此，我们用一个冻干的非洲爪蟾卵母细胞（这是一个单细胞）来展示该技术。使用C - 60（+）离子束，并结合计算机控制的分析和蚀刻周期。从表面获得的质谱在正离子模式下显示出对应于胆固醇（m/z 369）以及m/z 540 - 570和800 - 1000处其他脂质的峰，在负离子模式下显示出脂质脂肪酸侧链（例如，m/z 255）的峰。据我们所知，这是首次在实际生物系统中使用TOF - SIMS进行三维生物分子成像的展示。
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) using buckminsterfullerene (C-60) as the primary ion source has the ability to generate chemical images of surfaces with high sensitivities and minimal chemical damage. We studied the application of C-60(+) to depth profile a biological cell surface in a controlled manner and to subsequently image the revealed subsurfaces, in order to generate three-dimensional molecular images of the biological system. Such an analytical tool not only enables the surface localization of molecular species to be mapped but also enables the biomolecular distribution as a function of depth to be investigated with minimal sample preparation/intervention. Here we demonstrate the technique with a freeze-dried Xenopus laevis oocyte, which is a single cell. A C-60(+) ion beam was used with computer-controlled analyses and etch cycles. Mass spectra derived from the surface revealed peaks corresponding to cholesterol (m/z 369) and other lipids at m/z 540-570 and 800-1000, in the positive ion mode, and lipid fatty acid side chains (e.g., m/z 255) in the negative ion mode. To our knowledge, this is the first demonstration of the 3D biomolecular imaging within an actual biological system using TOF-SIMS.