Stimulatory effect of insulin-like growth factor-I on [3H]thymidine incorporation, DNA content and insulin biosynthesis and secretion of isolated pancreatic rat islets.
Stimulatory effect of insulin-like growth factor-I on [3H]thymidine incorporation, DNA content and insulin biosynthesis and secretion of isolated pancreatic rat islets.
复制标题
胰岛素样生长因子-I 对离体大鼠胰岛的 [3H] 胸苷掺入、DNA 含量以及胰岛素生物合成和分泌的刺激作用。
作者：
J. Sieradzki;H. Fleck;A. Chatterjee;H. Schatz
本文研究了胰岛素样生长因子-I（IGF-I）对大鼠胰岛生长和功能的影响。作为胰岛细胞复制的量度，同时估计[3 H]胸苷掺入和DNA含量作为（原）胰岛素生物合成和分泌的参数，作为B细胞功能能力的量度。在组织溶解后测量[3 H]胸苷的掺入;用荧光法测定DNA。（Pro-）胰岛素生物合成通过在免疫沉淀后掺入[3 H]亮氨酸并与蛋白A-Sepharose结合来测定。用IGF-I培养胰岛后，[3 H]胸苷掺入和DNA含量均显著增加。IGF-I还导致分离的胰岛的胰岛素生物合成和分泌增加。这些结果表明IGF-I在调节胰岛生长和功能中的作用。
The influence of insulin-like growth factor-I (IGF-I) on both the growth and function of isolated pancreatic rat islets was studied. As a measure of islet cell replication, [3H]thymidine incorporation and DNA content was estimated at the same time as parameters for (pro)insulin biosynthesis and secretion as a measure of the functional capacity of B cells. Incorporation of [3H]thymidine was measured after tissue solubilization; DNA was determined fluorometrically. (Pro-)insulin biosynthesis was determined by incorporation of [3H]leucine after immunoprecipitation and binding to protein A-Sepharose. There was a significant increase in both [3H]thymidine incorporation and DNA content after the culture of islets with IGF-I. IGF-I resulted also in an increase in insulin biosynthesis and secretion from isolated pancreatic islets. These results demonstrate a role of IGF-I in regulating the growth and function of pancreatic islets.