Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses
Mapping stripe rust resistance gene YrZH22 in Chinese wheat cultivar Zhoumai 22 by bulked segregant RNA-Seq (BSR-Seq) and comparative genomics analyses
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DOI：
10.1007/s00122-017-2950-0
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发表时间：
2017-07
影响因子：
5.4
通讯作者：
Yong Wang;Jingzhong Xie;Huaizhi Zhang;B. Guo;S. Ning;Yongxing Chen;P. Lu;Qiuhong Wu;Miaomiao Li-M
中科院分区：
文献类型：
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作者：
Yong Wang;Jingzhong Xie;Huaizhi Zhang;B. Guo;S. Ning;Yongxing Chen;P. Lu;Qiuhong Wu;Miaomiao Li-M
利用BSR-Seq和比较基因组学技术将抗条锈病基因YrZH 22定位于小麦4 BL 1染色体上一个4 Mb物理基因组区域，遗传距离为5.92 cM。小麦赤霉病（PST）是小麦上最具毁灭性的病害之一，严重威胁着世界小麦生产。中国小麦品种周麦22对目前流行的小麦品种CYR 34（V26）具有很高的抗性。对F5：6和F6：7重组自交系（RIL）群体的遗传分析表明，周麦22的成株抗条锈性受单基因控制，暂定名为YrZH 22。应用混合分离RNA-Seq（BSR-Seq）技术，筛选出7个SNP标记，SNP定位结果表明YrZH 22位于染色体臂4 BL上标记WGGB 105和WGGB 112之间。利用中国春4 BL基因组拼接图和粗山羊草4DL物理图谱的相应基因组区域进行比较基因组学分析，筛选出9个新的多态性标记，构建了粗山羊草YrZH 22的高分辨率遗传连锁图谱。YrZH 22位于标记WGGB 133和WGGB 146之间，遗传距离为5.92 cM，与中国春4 BL的4.1Mb基因组区间和Ae的2.2Mb基因组正向同源区相对应。tauschii 4DL. YrZH 22的遗传连锁图谱为YrZH 22的精细定位和定位克隆奠定了基础，并可用于小麦育种的分子标记辅助选择。
Key messageA stripe rust resistance gene YrZH22 was mapped by combined BSR-Seq and comparative genomics analyses to a 5.92 centimorgan (cM) genetic interval spanning a 4 Mb physical genomic region on wheat chromosome 4BL1.AbstractStripe rust, caused byPuccinia striiformisf. sp.tritici(PST), is one of the most destructive diseases of wheat and severely threatens wheat production worldwide. The widely grown Chinese wheat cultivar Zhoumai 22 is highly resistant to the current prevailing PST race CYR34 (V26). Genetic analysis of F5:6and F6:7recombinant inbred line (RIL) populations indicated that adult-plant stripe rust resistance in Zhoumai 22 is controlled by a single gene, temporarily designatedYrZH22. By applying bulked segregant RNA-Seq (BSR-Seq), 7 SNP markers were developed and SNP mapping showed thatYrZH22is located between markersWGGB105andWGGB112on chromosome arm 4BL. The corresponding genomic regions of the Chinese Spring 4BL genome assembly and physical map ofAegilops tauschii4DL were selected for comparative genomics analyses to develop nine new polymorphic markers that were used to construct a high-resolution genetic linkage map ofYrZH22.YrZH22was delimited in a 5.92 cM genetic interval between markersWGGB133andWGGB146, corresponding to 4.1 Mb genomic interval in Chinese Spring 4BL and a 2.2 Mb orthologous genomic region inAe. tauschii4DL. The genetic linkage map ofYrZH22will be valuable for fine mapping and positional cloning ofYrZH22, and can be used for marker-assisted selection in wheat breeding.