STRUCTURE AND EXPRESSION OF A MANDUCA-SEXTA LARVAL CUTICLE GENE HOMOLOGOUS TO DROSOPHILA CUTICLE GENES
STRUCTURE AND EXPRESSION OF A MANDUCA-SEXTA LARVAL CUTICLE GENE HOMOLOGOUS TO DROSOPHILA CUTICLE GENES
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DOI：
10.1016/0022-2836(88)90009-5
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发表时间：
1988-09-20
影响因子：
5.6
通讯作者：
RIDDIFORD, LM
中科院分区：
文献类型：
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作者：
REBERS, JE;RIDDIFORD, LM
从烟草天蛾Manduca sexta中分离出一个基因组克隆，因为它与果蝇幼虫角质层基因相似。RNA分析表明，这个克隆，B311，是在适当的时间表达幼虫角质层基因。使用B311 DNA的杂交选择实验表明，它编码14 ×103 Mr蛋白质。LCP-14，它是由一种抗Manduca幼虫角质层的抗血清沉淀的。我们对LCP-14基因的基因组和cDNA克隆进行了测序。对该cDNA序列的概念性翻译表明，LCP-14蛋白不仅与另一种Manduca角质层蛋白相似，而且与果蝇、肉蝇属和透明杯孢属角质层蛋白相似。由于这些蛋白质存在于柔性角质层中，并且具有相似的序列，我们推断它们是由同源基因编码的。
A genomic clone was isolated from the tobacco hornworm, Manduca sexta, by virtue of its similarity to a Drosophila larval cuticle gene. RNA analysis shows that this clone, B311, is expressed at times appropriate for a larval cuticle gene. Hybrid-selection experiments using B311 DNA show that it encodes a 14 .times. 103 Mr protein. LCP-14, which is precipitated by an antiserum to Manduca larval cuticle. We have sequenced both genomic and cDNA clones for the LCP-14 gene. A conceptual translation of the cDNA sequence shows that the LCP-14 protein is simlar not only to another Manduca cuticle protein, but also to Drosophila, Sarcophaga and Hyalophora cecropia cuticle proteins. Since these proteins are found in flexible cuticle and have similar sequences, we conclude they are encoded by homologous genes.