VARIATION IN THE GLUCOSINOLATE CONTENT OF OILSEED RAPE (BRASSICA-NAPUS L) LEAVES .2. RESPONSE TO INFECTION BY ALTERNARIA-BRASSICAE (BERK) SACC
VARIATION IN THE GLUCOSINOLATE CONTENT OF OILSEED RAPE (BRASSICA-NAPUS L) LEAVES .2. RESPONSE TO INFECTION BY ALTERNARIA-BRASSICAE (BERK) SACC
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DOI：
10.1111/j.1744-7348.1991.tb05648.x
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发表时间：
1991-04-01
影响因子：
2.6
通讯作者：
WALLSGROVE, R
中科院分区：
文献类型：
--
作者：
DOUGHTY, KJ;PORTER, AJR;WALLSGROVE, R
硫代葡萄糖苷被认为有助于十字花科植物对病虫害的抗性，包括油菜（甘蓝型油菜），并且已知在各种害虫侵染后会在芸薹属植物组织中积累。本研究调查了接种黑斑病病原体（芸薹链格孢）后油菜叶片中硫代葡萄糖苷浓度的变化。在接种后的20天内每隔一段时间采集单低品种Bienvenu（芥酸含量低）和双低品种Cobra（芥酸和硫代葡萄糖苷含量低）的第4叶和第6叶，并用高效液相色谱法分析硫代葡萄糖苷含量。两个品种接种叶片中的硫代葡萄糖苷都有积累，但Bienvenu品种的积累量更大，尤其是在第6叶。在硫代葡萄糖苷中，脂肪族化合物在Bienvenu品种中快速积累，但后来下降。吲哚基和芳香族硫代葡萄糖苷在两个品种中都有积累，但速度比脂肪族硫代葡萄糖苷慢。接种后单个硫代葡萄糖苷的积累程度存在差异。最初，Cobra品种的病害症状比Bienvenu品种更广泛，但在实验结束时，两个品种相应叶片上的症状相似。然而，第6叶的病斑明显少于第4叶。感染油菜中硫代葡萄糖苷的积累可能会限制现有真菌感染的传播或抑制后续的侵染尝试，尤其是在较嫩的叶片中。
The glucosinolates are thought to contribute to resistance to pests and diseases in members of the Cruciferae, including oilseed rape (Brassica napus) and they are known to accumulate in Brassica tissues after infestation by various pests. The present study investigated the changes in glucosinolate concentration in leaves of oilseed rape following inoculation with the dark leaf spot pathogen (Alternaria brassicae). Fourth and sixth leaves of the single-low cultivar Bienvenu (low in erucic acid) and the double-low cultivar Cobra (low in erucic acid and glucosinolate) were removed at intervals up to twenty days after inoculation and analysed for glucosinolate content using HPLC. Glucosinolates accumulated in inoculated leaves of both cultivars but the accumulation was greater for cv. Bienvenu, especially in sixth leaves. Among the glucosinolates, aliphatic compounds accumulated rapidly in cv. Bienvenu, but later declined. Indolyl and aromatic glucosinolates accumulated in both cultivars, but at a slower rate than the aliphatic glucosinolates. There were differences in the extent to which individual glucosinolates accumulated after inoculation. Disease symptoms were initially more extensive on cv. Cobra than on cv. Bienvenu but were similar on corresponding leaves of the two cultivars by the end of the experiment. However, sixth leaves had significantly less lesioning than fourth leaves. Glucosinolate accumulation in infected oilseed rape may restrict the spread of existing fungal infection or inhibit subsequent attempted infections, especially in younger leaves.