Transcriptomic analysis shows the antifungal mechanism of honokiol against Aspergillus flavus
Transcriptomic analysis shows the antifungal mechanism of honokiol against Aspergillus flavus
复制标题
转录组分析显示和厚朴酚抗黄曲霉的抗真菌机制
DOI：
10.1016/j.ijfoodmicro.2022.109972
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发表时间：
2022
影响因子：
5.4
通讯作者：
Yuansen Hu
中科院分区：
文献类型：
--
作者：
Wei Zhang;Bangbang Li;Yangyong Lv;Shan Wei;Shuaibing Zhang;Yuansen Hu
近年来，植物源性物质作为抗真菌剂受到了相当大的关注，以减少化学杀菌剂在食品防腐剂中的使用。厚朴酚类化合物和厚朴酚在100 μg/mL浓度下对黄曲霉孢子萌发和菌丝生长有抑制作用。此外，致病性试验表明，和厚朴对A.通过抑制分生孢子的产生来抑制玉米粉中的黄曲霉。采用荧光染色、透射电镜和生化分析等方法研究其对A.黄色的结果表明，和厚朴对A.黄曲霉毒素可能与质膜透性增加、ATP酶活性抑制、线粒体功能障碍和活性氧积累有关。此外，转录组学分析表明，和厚朴处理导致1578个不同的表达基因。Gen Ontology和京都基因和基因组百科全书分析表明，A.与孢子发育、细胞壁和膜的完整性、氧化应激和能量代谢相关的黄曲霉素表达显著下调。此外，通过定量实时聚合酶链反应验证了RNA-seq结果。我们的发现增强了对和厚朴的抗真菌活性和分子水平上的潜在作用机制的理解，支持和厚朴作为预防A.黄色的
Plant-derived substances as antifungal agents have received considerable attention in recent years to reduce the use of chemical fungicides in food preservatives. In this study, honokiol, a type of phenolic compound in Magnolia officinalis, was found to inhibit spore germination and mycelia growth of Aspergillus flavus at 100 μg/mL. In addition, a pathogenicity assay showed that honokiol had potent antifungal activity against A. flavus in corn flour by suppressing conidia production. Fluorescence staining, transmission electron microscopy and biochemical assays were performed to explore its possible inhibition mechanisms against A. flavus. The results showed that the destructive effect of honokiol on A. flavus appeared to be related to increased plasma membrane permeability, the inhibition of ATPase activity, mitochondrial dysfunction and the accumulation of reactive oxygen species. Furthermore, a transcriptomic analysis showed that honokiol treatment resulted in 1578 different expressed genes. Gen Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that some genes of A. flavus related to spore development, integrity of the cell wall and membrane, oxidative stress and energy metabolism were significantly downregulated. In addition, RNA-seq results were validated by quantitative real-time polymerase chain reactions. Our finding enhanced the understanding of the antifungal activity of honokiol and underlying mechanisms of action at the molecular level, supporting honokiol as a potential agent in preventing contamination by A. flavus.