SYNTHESIS, CONFORMATION AND HYDROLYTIC STABILITY OF P1,P3-DINUCLEOSIDE TRIPHOSPHATES RELATED TO MESSENGER-RNA 5'-CAP, AND COMPARATIVE KINETIC-STUDIES ON THEIR NUCLEOSIDE AND NUCLEOSIDE MONOPHOSPHATE ANALOGS
SYNTHESIS, CONFORMATION AND HYDROLYTIC STABILITY OF P1,P3-DINUCLEOSIDE TRIPHOSPHATES RELATED TO MESSENGER-RNA 5'-CAP, AND COMPARATIVE KINETIC-STUDIES ON THEIR NUCLEOSIDE AND NUCLEOSIDE MONOPHOSPHATE ANALOGS
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DOI：
10.1080/07328319008045191
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发表时间：
1990-01-01
影响因子：
1.3
通讯作者：
LONNBERG, H
中科院分区：
文献类型：
--
作者：
DARZYNKIEWICZ, E;STEPINSKI, J;LONNBERG, H
P1,P3-Dinucleoside triphosphates N(5'') ppp (5'') G, have been prepared in which N is 7-Me, 7-Et-, 7-Bn, N2, 7-diMe- or N2,N2,7-triMe-guanosine. Conformations of the nucleoside moieties have been determined and compared with those of the corresponding nucleoside monophosphates. The hydrolytic stability of the 7-alkylguanine ring has been studied and the origin of the structural effects elucidated by comparative kinetic studies with monomeric nucleoside and nucleotide analogs. The mechanism of the alkaline decomposition has been established by following the cleavageof 7-methylguanosine by 1H and 13C NMR spectroscopy.
