HOXC10 up-regulation contributes to human thyroid cancer and indicates poor survival outcome
HOXC10 up-regulation contributes to human thyroid cancer and indicates poor survival outcome
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DOI：
10.1039/c5mb00253b
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发表时间：
2015-01-01
影响因子：
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通讯作者：
Peng, Yongde
中科院分区：
文献类型：
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作者：
Feng, Xiaoyun;Li, Tuo;Peng, Yongde
HOX 基因已被充分描述为发育和形态发生以及最近的癌发生中的重要参与者。含同源结构域的基因 10 (HOXC10) 在人类甲状腺癌中的作用和临床意义尚不清楚。采用实时PCR和生物信息学分析方法检测上海人民医院（上海第一人民医院）正常和人甲状腺癌样本以及TCGA数据集中HOXC10的表达情况。还检测了人类甲状腺癌患者的生存时间。使用细胞计数试剂盒8（CCK8）分析检测细胞增殖，并通过流式细胞术评估细胞周期。通过transwell分析检测迁移和侵袭。采用基因集富集分析（GSEA）探讨HOXC10与信号通路的相关性。采用实时荧光定量PCR和Western blot分析检测人甲状腺癌细胞中信号通路相关基因的表达情况。上海人民医院HOXC10表达量和TCGA数据集显示，人类甲状腺癌组织中HOXC10表达量显着增加，且其表达量与高龄、不良病理分期、不良预后呈正相关。 shRNA 敲低 HOXC10 会阻断细胞周期并抑制迁移和侵袭。 TCGA 数据集中的 GSEA 显示 HOXC10 表达与细胞因子-细胞因子受体相互作用和趋化因子信号通路呈正相关。我们的数据表明，抑制 HOXC10 可能是人类甲状腺癌治疗的一种治疗策略。本研究探讨 HOXC10 在人类甲状腺癌中的作用和临床意义。
HOX genes have been well described as important players in development and morphogenesis, and more recently, in carcinogenesis. The role and clinical implication of homeodomain-containing gene 10 (HOXC10) in human thyroid cancer is poorly understood. Real-time PCR and bioinformatics analysis were used to detect the expression of HOXC10 in normal and human thyroid cancer samples from Shanghai General Hospital (also known as Shanghai First People's Hospital) and the TCGA dataset. The survival time of patients with human thyroid cancer was also detected. Cell Count Kit-8 (CCK8) analysis was used to detect cell proliferation, and the cell cycle was assessed by flow cytometry. Migration and invasion were detected by transwell analysis. Gene set enrichment analysis (GSEA) was used to explore the correlation of HOXC10 with signaling pathways. Real-time PCR and Western blot analysis were used to detect the expression of signaling pathway related genes in human thyroid cancer cells. HOXC10 expression in Shanghai General Hospital and the TCGA dataset revealed a significant increase in human thyroid cancer tissues and its expression was positively correlated with the advanced age, poor pathologic stage, and poor prognosis. HOXC10 knockdown by shRNA conferred cell cycle blocking and inhibition of migration and invasion. GSEA in the TCGA datasets revealed that HOXC10 expression was positively correlated with cytokine-cytokine receptor interaction and chemokine signaling pathways. Our data suggest that inhibition of HOXC10 may be a therapeutic strategy for human thyroid cancer treatment. This study investigates the role and clinical implication of HOXC10 in human thyroid cancer.