D-Glucose sensing by a plasma membrane regulator of G signaling protein, AtRGS1.

D-Glucose sensing by a plasma membrane regulator of G signaling protein, AtRGS1.
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DOI:
10.1016/j.febslet.2008.08.038
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发表时间:
2008-10-29
期刊:
影响因子:
3.5
通讯作者:
Jones, Alan M.
Jones, Alan M.
中科院分区:
生物学3区
文献类型:
--
作者:
Grigston, Jeffery C.;Osuna, Daniel;Scheible, Wolf-Ruediger;Liu, Chenggang;Stitt, Mark;Jones, Alan M.

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植物使用糖作为信号分子,并具有检测和响应糖可用性变化的机制,范围从二级信号分子水平到改变的基因转录。G蛋白偶联途径参与植物中的糖信号传导。拟南芥G蛋白信号蛋白调节因子1(AtRGS 1)将受体样七跨膜结构域与RGS结构域结合,以D-葡萄糖调节的方式与拟南芥Gα亚基(AtGPA 1)相互作用,并刺激AtGPA 1 GTPase活性。我们确定AtRGS 1与此处遗传定义的其他组分相互作用,作为D-葡萄糖的质膜传感器。AtRGS 1和AtGPA 1之间的这种相互作用部分涉及AtRGS 1的七跨膜结构域。
Plants use sugars as signaling molecules and possess mechanisms to detect and respond to changes in sugar availability, ranging from the level of secondary signaling molecules to altered gene transcription. G-protein-coupled pathways are involved in sugar signaling in plants. The Arabidopsis thaliana Regulator of G-protein Signaling protein 1 (AtRGS1) combines a receptor-like seven transmembrane domain with an RGS domain, interacts with the Arabidopsis Gα subunit (AtGPA1) in a D-glucose-regulated manner, and stimulates AtGPA1 GTPase activity. We determined that AtRGS1 interacts with additional components, genetically defined here, to serve as a plasma membrane sensor for D-glucose. This interaction between AtRGS1 and AtGPA1 involves, in part, the seven-transmembrane domain of AtRGS1.
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