Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.
Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.
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DOI：
10.1371/journal.pone.0160387
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Monk PN
中科院分区：
文献类型：
--
作者：
Ventress JK;Partridge LJ;Read RC;Cozens D;MacNeil S;Monk PN
金黄色葡萄球菌是皮肤和伤口感染的主要病原体之一。由于细菌粘附对于感染是必不可少的，阻断这一步骤可以减少病原体对宿主组织的入侵。基于宿主膜蛋白家族四跨膜蛋白的抗粘附疗法已经被开发，其可以抑制S.金黄色葡萄球菌对人体细胞的作用。测试了源自角质形成细胞表达的四跨膜蛋白CD9的合成肽的抗粘附特性，并且在低纳摩尔浓度下显示出抑制细菌粘附至培养的角质形成细胞，并且在人类皮肤感染的组织工程化模型中有效。这些潜在的治疗剂对角质形成细胞的活力、迁移或增殖没有影响，表明它们可能是目前皮肤感染治疗的有价值的补充。
Staphylococcus aureus is one of the primary causative agents of skin and wound infections. As bacterial adherence is essential for infection, blocking this step can reduce invasion of host tissues by pathogens. An anti-adhesion therapy, based on a host membrane protein family, the tetraspanins, has been developed that can inhibit the adhesion of S. aureus to human cells. Synthetic peptides derived from a keratinocyte-expressed tetraspanin, CD9, were tested for anti-adhesive properties and at low nanomolar concentrations were shown to inhibit bacterial adhesion to cultured keratinocytes and to be effective in a tissue engineered model of human skin infection. These potential therapeutics had no effect on keratinocyte viability, migration or proliferation, indicating that they could be a valuable addition to current treatments for skin infection.