Monitoring single-molecule reactivity on a carbon nanotube
Monitoring single-molecule reactivity on a carbon nanotube
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DOI：
10.1021/nl0724079
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发表时间：
2008-01-01
期刊：
影响因子：
10.8
通讯作者：
Collins, Philip G.
中科院分区：
文献类型：
--
作者：
Goldsmith, Brett R.;Coroneus, John G.;Collins, Philip G.
使用点功能化的碳纳米管设备，我们展示了对单个羧酸基与其直接环境相互作用的连续、多小时监测。在羧酸盐选择性试剂1-乙基-3-(3-二甲氨基丙基)碳二亚胺盐酸盐(EDC)存在下，纳米管器件的电导直接转换单分子连接和分离。由于该装置中只存在一种羧酸盐，因此可以通过数百个反应对其进行研究，从而提供了统计精度来直接确定羧基-EDC络合物的12 S寿命。该络合物的另一个不稳定性被实时传递，并被观察到具有2ms的中位寿命。通过确定与整体测量很好地一致的周转时间，并同时照亮额外的动力学，这些结果展示了该平台在单分子研究中补充光学方法的潜力。
Using point-functionalized carbon nanotube devices, we demonstrate continuous, multihour monitoring of a single carboxylate group interacting with its immediate environment. The conductance of the nanotube device directly transduces single-molecule attachments and detachments in the presence of the carboxylate-selective reagent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). Because only one carboxylate is present in the device, it can be studied through hundreds of reactions, providing the statistical accuracy to directly determine a 12 s lifetime of the carboxy-EDC complex. An additional instability of the complex is transduced in real time and observed to have a median lifetime of 2 ms. By determining a turnover time in good agreement with bulk measurements and simultaneously illuminating additional dynamics, these results demonstrate this platform's potential for complementing optical methods in single-molecule research.