Oropharyngeal carcinoma in non-smokers and non-drinkers: A role for HPV
Oropharyngeal carcinoma in non-smokers and non-drinkers: A role for HPV
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DOI：
10.1016/j.oraloncology.2008.07.008
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发表时间：
2009-06-01
期刊：
影响因子：
4.8
通讯作者：
Webster-Cyriaque, Jennifer
中科院分区：
文献类型：
--
作者：
Andrews, Elizabeth;Seaman, William T.;Webster-Cyriaque, Jennifer
从1973年到2001年，口咽鳞状细胞癌（OSCC）的发病率每年增加3%。口腔鳞状细胞癌可归因于烟草和酒精，但25%与典型风险无关。在非吸烟/非饮酒（NS/ND）OSCC患者中进行HPV检测的病例对照研究以前没有进行过。本研究的主要目的是确定高危型HPV感染是否与非吸烟者/非饮酒者口腔鳞状细胞癌的发生显著相关。利用病例对照设计，连续确定了18例癌症病例和22例良性活检对照。对活检组织进行（i）HPV-L1共有PCR和测序（ii）实时PCR。Logistic回归分析显示，病例组肿瘤中HPV感染的可能性是对照组的6.1倍（OR 95%CI，1.3 ~ 28）。通过一致性和实时PCR，在14/18例病例和6/22例对照的扁桃体和舌根（口咽）中容易检测到高危HPV-DNA。在含有高危HPV的病变中，85%（17/20）起源于口咽部（卡方检验，p = 0.03）。在30%（3/10）的先前患有口腔癌的个体中，在口咽部的良性活检中也检测到高危HPV（卡方，p = 0.006）JNS/ND中的OSCC的感染性质通过HPV的一致检测而揭示，这表明HPV在转化口腔上皮中的潜在作用，提供了需要在NS/ND中筛查口咽部的HPV的进一步证据。(C)2008年由Elsevier Ltd.出版
Incidence of oropharyngeal squamous cell carcinoma (OSCC) increased 3% annually from 1973 to 2001. OSCC's can be attributed to tobacco and alcohol, but 25% are unlinked to typical risks. Case-control studies on HPV detection in non-smoking/non-drinking (NS/ND) OSCC patients have not previously been performed. The primary objective of this study was to determine whether high-risk HPV infection was significantly associated with development of oral squamous malignancy in non-smokers/non-drinkers.A chart review of 802 OSCC patients from the UNC Pathology Archives (1995-2006) yielded 40 non-smoker/non-drinker subjects. Utilizing a case-control design, 18 cancer cases and 22 benign biopsy controls were consecutively identified. Biopsy tissue was subjected to (i) HPV-L1 consensus PCR and sequencing (ii) real-time PCR. Chi-square and logistic regression analysis was employed.Logistic regression analysis determined that cases were 6.1 (OR 95% CI, 1.3-28) times more likely to have HPV infection in their tumors than controls. High-risk HPV-DNA was readily detected in the tonsils and base of tongue (oropharynx) of 14/18 cases and 6/22 controls by both consensus and real-time PCR. Of high-risk HPV containing lesions, 85% (17/20) originated in the oropharynx (chi-square, p = 0.03). High risk HPV was also detected in benign biopsies of the oropharynx in 30% (3/10) of individuals who had a previous oral cancer (chi-square, p = 0.006).The infectious nature of OSCC in NS/ND was revealed by consistent detection of HPV, suggesting HPV's potential role in transforming oral epithelium, providing further evidence of the need to screen the oropharynx for HPV in NS/ND. (C) 2008 Published by Elsevier Ltd.