Characterization of a new megakaryocytic cell line: the Dami cell.
Characterization of a new megakaryocytic cell line: the Dami cell.
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DOI：
10.1182/blood.v72.6.1968.bloodjournal7261968
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发表时间：
1988-12
期刊：
影响因子：
20.3
通讯作者：
S. Greenberg;Rosenthal Ds;T. A. Greeley;R. Tantravahi;R. Handin
中科院分区：
文献类型：
--
作者：
S. Greenberg;Rosenthal Ds;T. A. Greeley;R. Tantravahi;R. Handin
从一名巨核细胞白血病患者的血液中建立了一种新的人类巨核细胞系(Dami)。Dami细胞主要在悬浮液中生长，倍增时间为24-30小时。在光镜和电子显微镜下，Dami细胞的直径从12微米到120微米不等，具有巨核细胞特有的分叶状核。至少89%的细胞与抗血小板膜糖蛋白(GP)Ib、IIb/IIIa和血糖素的单抗反应。这些细胞不与抗淋巴、单核细胞、粒细胞或巨噬细胞抗原的抗体反应。13%的细胞成为多倍体，自发达到4N DNA倍体水平。佛波酯(PMA)处理后，倍性水平大于4N的细胞比例增加了3倍，并可分为不同的倍性组。PMA还增加了GPIB、GPIIb/GPIIa复合体、L和von Willebrand因子的表达。细胞遗传学分析显示，这是一种人类男性超二倍体核型，其模式染色体数目为54对，并有几条一致的克隆性染色体异常。其中包括5号染色体的部分缺失和3号染色体的易位。与其他只有一小部分细胞表达巨核细胞表型的其他巨核细胞株不同，几乎所有的Dami细胞都表达血小板糖蛋白。因此，Dami细胞为研究人类巨核细胞的生化和分化提供了一个较好的模型。
A new human megakaryocytic cell line (Dami) has been established from the blood of a patient with megakaryoblastic leukemia. The Dami cells grow primarily in suspension with a doubling time of 24 to 30 hours. By light and electron microscopy, the Dami cells range in size from 12 to 120 micron in diameter and have lobulated nuclei characteristic of megakaryocytes. At least 89% of the cells react with monoclonal antibodies against platelet glycoproteins (GP) Ib and IIB/IIIa, and glycophorin. The cells do not react with antibodies against lymphoid, monocyte, granulocyte, or macrophage antigens. Thirteen percent of the cells become polyploid, spontaneously achieving greater than 4N DNA ploidy levels. In response to phorbol myristate acetate (PMA), the proportion of cells with ploidy levels greater than 4N increased threefold and could be separated into discrete ploidy groups. PMA also increased the expression of GPIb, the GPIIb/GPIIIa complex,l and von Willebrand factor. Cytogenetic analysis revealed a human male hyperdiploid karyotype with a modal chromosome number of 54 to 64 and several consistent clonal chromosomal abnormalities. These included a partial deletion of chromosome 5 and a translocation involving chromosome 3. In contrast to other megakaryocytic cell lines in which only a small portion of the cells express the megakaryocytic phenotype, nearly all of the Dami cells express platelet glycoproteins. Thus, the Dami cells provide a superior model in which to study human megakaryocyte biochemistry and differentiation.