Triggers of the HSP70 stress response: environmental responses and laboratory manipulation in an Antarctic marine invertebrate (Nacella concinna)
Triggers of the HSP70 stress response: environmental responses and laboratory manipulation in an Antarctic marine invertebrate (Nacella concinna)
复制标题
DOI：
10.1007/s12192-009-0117-x
复制
发表时间：
2009-11-01
影响因子：
3.8
通讯作者：
Peck, Lloyd S.
中科院分区：
文献类型：
--
作者：
Clark, Melody S.;Peck, Lloyd S.
南极帽贝，Nacella concinna，表现出经典的热休克反应，与上调的诱导热休克蛋白70（HSP 70）基因的复制形式，响应于海水温度的实验操作。然而，这种反应只发生在实验室中的温度远远超过任何经验的领域。随后对潮间带动物的环境采样也显示这些基因的上调，但温度阈值远低于在实验室中引起反应所需的温度阈值。据推测，这反映了在潮间带遇到的应力的复杂性。在这里，我们描述了进一步的一系列实验，包括实验室操作和环境采样的N。concinna。我们研究了HSP 70基因家族成员（HSP 70 A，HSP 70 B，GRP 78和HSC 70）的表达，以响应进一步的一套环境应激：季节性和实验性寒冷，淡水，干燥，慢性热和定期再现。较低的温度（-1.9A摄氏度和-1.6A摄氏度），通常会导致所有HSP 70家族成员的下调，当从冬季出现和海水温度升高时，HSC 70会上调。淡水浸泡无明显反应。在响应急性和慢性热处理加上模拟潮汐周期，数据显示了一个明确的模式。HSP 70 A对热表现出强烈但非常短期的反应，而复制的HSP 70 B也表现出热是触发因素，但对复杂的应激反应更持久。GRP 78的表达表明，它在这里描述的实验条件下作为一个广义的应激反应。HSC 70是响应于不同类型的长期应激而调用的主要伴侣。这些结果不仅为HSP 70基因表达对环境变化的复杂性提供了有趣的线索，而且还为非模式物种的应激反应提供了见解。
The Antarctic limpet, Nacella concinna, exhibits the classical heat shock response, with up-regulation of duplicated forms of the inducible heat shock protein 70 (HSP70) gene in response to experimental manipulation of seawater temperatures. However, this response only occurs in the laboratory at temperatures well in excess of any experienced in the field. Subsequent environmental sampling of inter-tidal animals also showed up-regulation of these genes, but at temperature thresholds much lower than those required to elicit a response in the laboratory. It was hypothesised that this was a reflection of the complexity of the stresses encountered in the inter-tidal region. Here, we describe a further series of experiments comprising both laboratory manipulation and environmental sampling of N. concinna. We investigate the expression of HSP70 gene family members (HSP70A, HSP70B, GRP78 and HSC70) in response to a further suite of environmental stressors: seasonal and experimental cold, freshwater, desiccation, chronic heat and periodic emersion. Lowered temperatures (-1.9A degrees C and -1.6A degrees C), generally produced a down-regulation of all HSP70 family members, with some up-regulation of HSC70 when emerging from the winter period and increasing sea temperatures. There was no significant response to freshwater immersion. In response to acute and chronic heat treatments plus simulated tidal cycles, the data showed a clear pattern. HSP70A showed a strong but very short-term response to heat whilst the duplicated HSP70B also showed heat to be a trigger, but had a more sustained response to complex stresses. GRP78 expression indicates that it was acting as a generalised stress response under the experimental conditions described here. HSC70 was the major chaperone invoked in response to long-term stresses of varying types. These results provide intriguing clues not only to the complexity of HSP70 gene expression in response to environmental change but also insights into the stress response of a non-model species.