Total cotinine in plasma: A stable biomarker for exposure to tobacco smoke
Total cotinine in plasma: A stable biomarker for exposure to tobacco smoke
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DOI：
10.1097/00004714-200210000-00009
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发表时间：
2002-10-01
影响因子：
2.9
通讯作者：
Crooks, PA
中科院分区：
文献类型：
--
作者：
de Leon, J;Diaz, FJ;Crooks, PA
血浆可替宁浓度测定是流行病学研究中用于量化吸烟和暴露于环境烟草烟雾的主要测定方法。然而，可替宁被生物转化为次级代谢产物。该初步研究测定了可替宁、可替宁葡糖苷酸、3-羟基可替宁和3-羟基可替宁葡糖苷酸的血浆浓度。通过所有四种代谢产物的总和测定总可替宁浓度。本研究的目的是（1）探索总可替宁浓度作为吸烟测量和非吸烟者暴露于环境烟草烟雾的测量的稳定性和有效性，（2）通过进行上午和下午测量，探索吸烟者中四种尼古丁代谢物中每一种的血浆浓度的稳定性，（3）探讨葡萄糖醛酸化指标作为酶活性指标的稳定性。受试者样本包括76名白色志愿者（32%吸烟者和68%非吸烟者）。血浆总可替宁浓度似乎非常稳定，表明总可替宁浓度可能是采用单一血浆样本进行流行病学研究的良好指标。此外，血浆总可替宁浓度也比血浆可替宁浓度更准确地反映了环境烟草烟雾的暴露，后者无法识别27%的被动吸烟者。3-羟基可替宁葡萄糖醛酸苷和3-羟基可替宁血浆浓度几乎与可替宁浓度一样稳定。然而，可替宁葡萄糖醛酸苷及其葡萄糖醛酸化指数不稳定，表明可替宁葡萄糖醛酸苷在体内发生解结合。需要在一天中使用两次以上的血液采集对血浆中的总可替宁进行新的研究，以明确确定它是流行病学研究的稳定生物标志物。
Determination of plasma cotinine concentration is the predominant assay employed to quantify smoking and exposure to environmental tobacco smoke in epidemiological studies. However, cotinine is biotransformed into secondary metabolites. This pilot study determined plasma concentrations of cotinine, cotinine glucuronide, 3-hydroxycotinine, and 3-hydroxycotinine glucuronide. Total cotinine concentration was determined by summation of all four metabolites. The goals of this study were (1) to explore the stability and validity of total cotinine concentration as a measure of tobacco smoking and as a measure of exposure to environmental tobacco smoke in nonsmokers, (2) to explore the stability of plasma concentrations of each of the four nicotine metabolites in smokers by performing a.m. and p.m. measures, and (3) to explore the stability of indices of glucuronidation as measures of possible markers for enzymatic activity. The subject sample included 76 white volunteers (32% smokers and 68% nonsmokers). Plasma total cotinine concentration appeared to be very stable, suggesting that total cotinine concentration may be a good measure for epidemiological studies employing a single plasma sample. Moreover, plasma total cotinine concentration also reflected exposure to environmental tobacco smoke more accurately than did plasma cotinine concentration, which would have not identified 27% of passive smokers.3-Hydroxycotinine glucuronide and 3-hydroxycotinine plasma concentrations were almost as stable as cotinine concentrations. However, cotinine glucuronide and its indices of glucuronidation were unstable, suggesting that cotinine glucuronide undergoes in vivo deconjugation. New studies of total cotinine in plasma using more than two blood collections during the day are needed to definitively establish that it is a stable biomarker for epidemiological studies.