CDP-choline treatment induces brain plasticity markers expression in experimental animal stroke
CDP-choline treatment induces brain plasticity markers expression in experimental animal stroke
复制标题
DOI：
10.1016/j.neuint.2011.12.015
复制
发表时间：
2012-02-01
影响因子：
4.2
通讯作者：
Diez-Tejedor, Exuperio
中科院分区：
文献类型：
--
作者：
Gutierrez-Fernandez, Maria;Rodriguez-Frutos, Berta;Diez-Tejedor, Exuperio
我们在实验动物模型中研究了CDP-胆碱对脑梗死急性期脑可塑性标志物表达的影响。雄性Sprague-Dawley大鼠进行永久性大脑中动脉闭塞（pMCAO），并从pMCAO后30分钟开始每天用CDP-胆碱（500 mg/kg）治疗或不治疗14天。功能状态用Roger试验评估;病变体积用磁共振成像（MRI）和苏木精和伊红染色（H & E）评估;细胞死亡用TUNEL评估;细胞增殖用BrdU免疫组织化学评估;血管内皮生长因子（VEGF）、突触素、胶质细胞酸性蛋白（GFAP）和低密度脂蛋白受体相关蛋白（LRP）用免疫荧光和Western印迹技术评估。CDP-胆碱显着改善功能恢复，并在14天的MRI，TUNEL阳性细胞数和LRP水平上减少病变体积。此外，CDP-胆碱显着增加BrdU，VEGF和突触素值和降低GFAP水平在梗死周围区相比，梗死组。总之，我们的数据表明，CDP胆碱改善永久性大脑中动脉闭塞后的功能恢复与减少病变体积，细胞死亡和LRP表达。事实上，CDP-胆碱在缺血性中风的梗塞周围区域中增加细胞增殖、血管发生和突触素水平，并降低GFAP水平。(C)2012爱思唯尔有限公司保留所有权利。
We investigated the effect of CDP-choline on brain plasticity markers expression in the acute phase of cerebral infarct in an experimental animal model. Male Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (pMCAO) and treated or not with CDP-choline (500 mg/kg) daily for 14 days starting 30 min after pMCAO. Functional status was evaluated with Roger's test; lesion volume with magnetic resonance imaging (MRI) and hematoxylin and eosin staining (H & E); cell death with TUNEL; cellular proliferation with BrdU immunohistochemistry; vascular endothelial growth factor (VEGF), synaptophysin, glial fibrillary acidic protein (GFAP) and low-density lipoprotein receptor-related protein (LRP) by immunofluorescence and Western-blot techniques. CDP-choline significantly improved functional recovery and decreased lesion volume on MRI, TUNEL-positive cell number and LRP levels at 14 days. In addition, CDP-choline significantly increased BrdU, VEGF and synaptophysin values and decreased GFAP levels in the peri-infarct zone compared with the infarct group. In conclusion, our data indicate that CDP-choline improved functional recovery after permanent middle cerebral artery occlusion in association with reductions in lesion volume, cell death and LRP expression. In fact, CDP-choline increased cell proliferation, vasculogenesis and synaptophysin levels and reduced GFAP levels in the peri-infarct area of the ischemic stroke. (C) 2012 Elsevier Ltd. All rights reserved.