DETECTION OF A SPECIFIC MITOCHONDRIAL-DNA DELETION IN TISSUES OF OLDER HUMANS
DETECTION OF A SPECIFIC MITOCHONDRIAL-DNA DELETION IN TISSUES OF OLDER HUMANS
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DOI：
10.1093/nar/18.23.6927
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发表时间：
1990-12-11
影响因子：
14.9
通讯作者：
ARNHEIM, N
中科院分区：
文献类型：
--
作者：
CORTOPASSI, GA;ARNHEIM, N
使用PCR，我们发现来自成年人个体的正常心肌和大脑含有低水平的特定线粒体DNa缺失，以前仅在受某些类型的神经肌肉疾病影响的患者中发现。 在胎仔心脏或脑中未观察到这种缺失。 实验测试支持这样的想法，即缺失存在于体内的成年线粒体中，而不是PCR的体外人工产物。 我们的数据提供了直接的实验支持的想法，线粒体DNA缺失的积累可能是重要的老化。
Using PCR, we found that normal heart muscle and brain from adult human individuals contain low levels of a specific mitochondrial DNa deletion, previously found only in patients affected with certain types of neuromuscular disease. This deletion was not observed in fetal heart or brain. Experimental tests support the idea that the deletion exists in vivo in adult mitochondria and is not an in vitro artifact of PCR. Our data provide direct experimental support for the idea that accumulation of mitochondrial DNA deletions may be important in aging.