Advances in gold nanoparticles for mycotoxin analysis
Advances in gold nanoparticles for mycotoxin analysis
复制标题
用于霉菌毒素分析的金纳米粒子的进展
作者：
Linxia Wu;Meng Wang;Dizhe Wei
霉菌毒素是真菌自然产生的有毒次生代谢物。由于食物中通常含有微量霉菌毒素，它们可引起人类和动物的各种急性和慢性疾病。因此，开发一种快速、灵敏的霉菌毒素检测技术非常重要。除了最初和经典的酶联免疫吸附测定（ELISA）外，近十年来还开发了一系列生物传感器来分析食品中的霉菌毒素，具有分析快速、简单、便携、重现性、稳定性、准确性和低成本的优点。纳米材料已被纳入生物传感器中，以在检测限、线性范围、分析稳定性、低生产成本等方面获得更好的分析性能。金纳米颗粒（AuNPs）是研究最广泛和最常用的纳米材料之一，可用作固定载体、信号放大器、介体和模拟酶标记。本文旨在全面概述 AuNPs 在通过 ELISA 和生物传感器检测霉菌毒素方面的最新进展。详细描述了检测方法及其应用原理，并讨论了当前的挑战和未来的前景。
Mycotoxins are toxic secondary metabolites naturally produced by fungi. They can cause various kinds of acute and chronic diseases in both humans and animals since food usually contains trace amounts of mycotoxins. Thus, it is important to develop a rapid and sensitive technique for mycotoxin detection. Except for the original and classical enzyme-linked immunosorbent assays (ELISA), a series of biosensors has been developed to analyze mycotoxins in food in the last decade with the advantages of rapid analysis, simplicity, portability, reproducibility, stability, accuracy, and low cost. Nanomaterials have been incorporated into biosensors for the purpose of achieving better analytical performance in terms of limit of detection, linear range, analytical stability, low production cost, etc. Gold nanoparticles (AuNPs) are one of the most extensively studied and commonly used nanomaterials, which can be employed as an immobilization carrier, signal amplifier, mediator and mimic enzyme label. This paper aims to present an extensive overview of the recent progress in AuNPs in mycotoxin detection through ELISA and biosensors. The details of the detection methods and their application principles are described, and current challenges and future prospects are discussed as well.