Histone genes are clustered but not tandemly repeated in the chicken genome.
Histone genes are clustered but not tandemly repeated in the chicken genome.
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组蛋白基因在鸡基因组中聚集但不串联重复。
DOI：
10.1073/pnas.78.5.2856
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发表时间：
1981
影响因子：
11.1
通讯作者：
Dodgson,JB
中科院分区：
文献类型：
--
作者：
Engel,JD;Dodgson,JB
以含有紫球海胆H2 a和H3基因的重组海胆DNA探针pSp 17为探针，对重组鸡DNA文库进行组蛋白基因的筛选。三个分离的λ重组体进行了分析，通过限制性内切酶图谱和Southern印迹法;一个组蛋白H3基因编码重组体进行了进一步分析，通过DNA序列测定。这些研究表明，鸡的组蛋白基因并不是串联重复的，但至少有几个组蛋白基因在鸡的基因组中以非重复的方式紧密相连。这种安排与果蝇和海胆的进化影响进行了讨论。
The recombinant chicken DNA library was screened for histone genes by using pSp17, a recombinant sea urchin DNA probe containing the H2a and H3 genes of Strongylocentrotus purpuratus. Three of the isolated lambda recombinants have been analyzed by restriction enzyme mapping and Southern blotting; one histone H3 gene-encoding recombinant was further analyzed by DNA sequence determination. These studies reveal that the chicken histone genes are not tandemly reiterated, but that, at the least, several histone genes are physically closely linked in a nonrepetitive arrangement within the chicken genome. The evolutionary implications of this arrangement versus that seen in Drosophila and sea urchins is discussed.