Mitochondrial DNA variation and RAPD mark oleasters, olive and feral olive from Western and Eastern Mediterranean
Mitochondrial DNA variation and RAPD mark oleasters, olive and feral olive from Western and Eastern Mediterranean
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DOI：
10.1007/s00122-002-0883-7
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发表时间：
2002-05-01
影响因子：
5.4
通讯作者：
Giannettini, J
中科院分区：
文献类型：
--
作者：
de Caraffa, VB;Maury, J;Giannettini, J
橄榄树（栽培和野生形式）的遗传多样性的研究可能有助于揭示野生种质的农艺性状，并试图了解橄榄树驯化的历史。以这种方式，从两个科西嘉岛和撒丁岛地中海岛屿的栽培和野生橄榄的核和线粒体DNA的研究进行了RAPD和RFLP标记。结果表明，利用基于Nei和Li相似性指数的UPGMA聚类分析，大部分品种和大部分油桐被分离。大多数的oleasters进行要么是ESTA或MCK的有丝分裂型，在地中海西部的橄榄的特点，而大多数的品种进行ME1的有丝分裂型，在地中海东部的橄榄的特点。结果表明，RAPD标记与有丝分裂型标记相结合，可作为区分野生型和西方真油鲶两大类群的有力工具。真正的oleasters的特点是西方的mitotype和西方的RAPD模式。野生型起源于变种或变种与沙枣的杂交。因此，正如预期的那样，其中一些与它们所衍生的品种聚集在一起。其他野生形式与oleasters聚类，仅通过其有丝分裂型测定检测。这项研究还使我们能够区分两个人口的种植橄榄在科西嘉：一个与密切的关系船舶与意大利品种（受东方）和一个选择当地oleasters可能是由于一个更好的本地适应比外国品种。
The study of genetic diversity within the olive-tree (cultivated and wild forms) may be useful to reveal agronomic traits in the wild germplasm and to try to understand the history of the olive-tree domestication. In this way, a study of nuclear and mitochondrial DNAs of cultivated and wild olives from two Corsican and Sardinian Mediterranean islands was performed using RAPD and RFLP markers. Our results show that most of the varieties and most of the oleasters were separated using the UPGMA dendrogram based on the Nei and Li similarity index. Most of oleasters carried either the MOM or MCK mitotype, characteristic of olives in the Western Mediterranean, whereas most of the varieties carried the ME1 mitotype, characteristic of olives in the East Mediterranean. The results indicate that the combination of mitotype and RAPD markers can be used as a powerful tool for differentiating two groups in the wild forms: the Western true oleasters and the feral forms. The true oleasters are characterized by a Western mitotype and a Western RAPD pattern. Feral forms originate either from varieties or from hybridisation between a variety and an oleaster. Consequently, as expected, some of them aggregated with the varieties from which they were derived. The other feral forms are clustered with the oleasters and were detected only by their mitotype determination. This study has also permitted us to, differentiate two populations of cultivated olives in Corsica: one with close relations ships with Italian varieties (influenced by the East) and one selected from local oleasters probably due to a better local adaptation than foreign varieties.